CD68 antibody human specific - HS-460 017

CD68 is a marker for macrophages and activated microglia
Rat monoclonal purified IgG
Cat. No.: HS-460 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-460 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 200 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: not tested yet
IHC-P: 1 : 100 gallery  
Clone 186F9B4
Subtype IgG2b (κ light chain)
Immunogen synthetic peptide corresponding to residues near the carboxy terminus of human CD68 (UniProt Id: P34810)
Reactivity Reacts with: human (P34810).
No signal: mouse (P31996).
Other species not tested yet.
Data sheet hs-460_017.pdf

References for CD68 - HS-460 017

Distinct tau neuropathology and cellular profiles of an APOE3 Christchurch homozygote protected against autosomal dominant Alzheimer's dementia.
Sepulveda-Falla D, Sanchez JS, Almeida MC, Boassa D, Acosta-Uribe J, Vila-Castelar C, Ramirez-Gomez L, Baena A, Aguillon D, Villalba-Moreno ND, Littau JL, et al.
Acta neuropathologica (2022) 1443: 589-601. HS-460 017 IHC-P; tested species: human
Cat. No.: HS-460 017
Amount: 200 µl
Price: $415.00

Indirect immunostaining of human tonsil section with rat anti-CD68 (red) and rabbit anti-IBA1 (green).

Distinct tau neuropathology and cellular profiles of an APOE3 Christchurch homozygote protected against autosomal dominant Alzheimer's dementia.
Sepulveda-Falla D, Sanchez JS, Almeida MC, Boassa D, Acosta-Uribe J, Vila-Castelar C, Ramirez-Gomez L, Baena A, Aguillon D, Villalba-Moreno ND, Littau JL, et al.
Acta neuropathologica (2022) 1443: 589-601. HS-460 017 IHC-P; tested species: human
Background

CD68, also called Lamp-4 in humans or macrosialin in mice, is a highly glycosylated type I transmembrane protein that belongs to the lysosome associated membrane protein (LAMP) family (1). CD68 expression is increased in cells associated with elevated phagocytic and degradative activity. High CD68 expression is detected in cells of the mononuclear phagocyte lineage including macrophages, osteoclasts, and myeloid dendritic cells (2). CD68 is a marker of activated microglia and only expressed at low levels in resting microglia (3). Staining for CD68 is predominantly intracellular, only 10 -15% of it is found on the cell surface. In oncology research, CD68 is the major biomarker for quantification of tumor-associated macrophages (TAMs) (4). High infiltration of CD68+ macrophages is an independent prognostic factor for overall survival in several tumor entities (5).

Protocols for HS-460 017