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Synaptophysin1 antibody - 101 308 K.O.

Synaptophysin is a major synaptic vesicle membrane protein and a useful neuroendocrine tumor marker
Guinea pig monoclonal recombinant IgG
Cat. No.: 101 308
Amount: 50 µg
Price: $415.00
Cat. No. 101 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 10000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 up to 1 : 5000 gallery  
Clone Gp7.2
Subtype IgG2 (κ light chain)
Immunogen Full-length rat Synaptophysin 1 recombinant protein (UniProt Id: P07825)
Epitop AA 219 to 307 from rat Synaptophysin1 (UniProt Id: P07825)
Reactivity Reacts with: human (P08247), rat (P07825), mouse (Q62277), mammals.
Weaker signal: zebrafish, other vertebrates.
Other species not tested yet.
Specificity K.O. validated
Remarks

This antibody is a chimeric antibody based on the well-known monoclonal mouse antibody clone 7.2. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Synaptophysin 1 is widely used as marker for nerve terminals and neuroendocrine tumors. For reasons still unknown, neuronal synaptophysin is better recognized than neuroendocrine synaptophysin. If this is a problem, the polyclonal rabbit antibody, cat. no. 101 002, is recommended.
Data sheet 101_308.pdf

References for Synaptophysin1 - 101 308

Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates.
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P
Nature communications (2023) 141: 455. 101 308 ICC; tested species: mouse
Cat. No.: 101 308
Amount: 50 µg
Price: $415.00
Synaptic vesicle proteins and ATG9A self-organize in distinct vesicle phases within synapsin condensates.
Park D, Wu Y, Wang X, Gowrishankar S, Baublis A, De Camilli P
Nature communications (2023) 141: 455. 101 308 ICC; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC
IHC-P
IHC-P
Background

Synaptophysin1, also referred to as p38-1, is a membrane glycoprotein of synaptic vesicles that is ubiquitously expressed in all neurons and in many endocrine cells. It is currently the most widely used marker for nerve terminals and probably the best marker for the pathologist in differentiating neuroendocrine tumors.
Synaptophysin1 has four transmembrane domains with both N- and C-terminus facing the cytoplasm. It binds to synaptobrevin1 and synaptobrevin2 in detergent extracts but its function has not been elucidated completely. It forms a complex with dynamin at high Ca2+ concentration suggesting an involvement in synaptic vesicle endocytosis. As typical for synaptic vesicle proteins, synaptophysin1 represents a small protein family with two additonal members, synaptoporin (synaptophysin2) and panthophysin. Like synaptophysin1, synaptoporin is widely expressed in neurons and colocalizes with synaptophysin1 on synaptic vesicles whereas panthophysin is expressed in all tissues.
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