HistoSure - Caveolin1

Background

Caveolae are distinct flask shaped invaginations which contain high concentrations of cholesterol and sphingolipids. These subcellular compartments can be found at the surface of many cell types.
Three isoforms (caveolin1, 2, 3) have been described so far. caveolin1 is the major coat protein of endothelial caveolae and a knock out of this protein leads to a complete loss of these cell surface structures in various cell types.
caveolin1 is also expressed in brain where it localizes to astrocytes, oligodendrocytes and endothelial cells but seems to be absent from neurons. Very recently a correlation of increased caveolin1 expression in brain and Alzheimer's disease has been described.

1 ) Kang MJ, Experimental & molecular medicine (2006) 382: 126-33.
2 ) Gaudreault SB, Neurobiology of aging (2004) 256: 753-9.
3 ) Virgintino D, Neuroscience (2002) 1151: 145-52.
4 ) Ikezu T, Brain research (1998) 8042: 177-92.
5 ) Cameron PL, The Journal of neuroscience : the official journal of the Society for Neuroscience (1997) 1724: 9520-35.

Cat. No. 161 011	100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP staining) gallery IP: not tested yet ICC: 1 : 500 (see remarks) gallery IHC: 1 : 500 (see remarks) gallery IHC-P: 1 : 1000 gallery IHC-Fr: 1 : 500 (see remarks) gallery IHC-G: 1 : 500 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on glyoxal fixed (IHC-G) tissue. The tissue is perfused with saline and afterwards immersion fixed with a glyoxal solution. For details of the glyoxal solution, please refer to the remarks section. Immunoreactivity is usually revealed by fluorescence.
Clone	SY-177E4
Subtype	IgG2b (κ light chain)
Immunogen	Recombinant protein corresponding to cytoplasatic domain of mouse Caveolin1 (UniProt Id: P49817)
Reactivity	Reacts with: mouse (P49817), rat (P41350), human (Q03135). Other species not tested yet.
Remarks	ICC: Methanol fixation is recommended. IHC: Heat-mediated antigen retrieval (citrate buffer pH 6 or Tris-EDTA buffer pH 9.0) is required for immunohistochemical staining. IHC-Fr: Formaldehyde, Methanol or Methanol-acetone (1:1 mix) fixation are recommended. IHC-G: 3% glyoxal, 1% acetic acid, 20% ethanol, in ddH₂O, pH 4.2-4.4, according to Richter et al. 2017 or 9% glyoxal, 8% acetic acid, in ddH₂O, pH 4.2-4.4, according to Konno et al. 2023 are recommended.
Data sheet	161_011.pdf

Caveolin1 antibody - 161 011