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Vimentin antibody - 172 002 K.O.

Vimentin is a type III intermediate filament
Rabbit polyclonal antiserum
Cat. No.: 172 002
Amount: 200 µl
Price: $355.00
Cat. No. 172 002 200 µl antiserum, lyophilized. For reconstitution add 200 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 200 up to 1 : 500 gallery  
IHC-P: 1 : 500 gallery  
EM: yes
Immunogen Recombinant protein corresponding to AA 1 to 466 from mouse Vimentin (UniProt Id: P20152)
Reactivity Reacts with: human (P08670), rat (P31000), mouse (P20152), monkey.
No signal: zebrafish.
Other species not tested yet.
Specificity K.O. validated PubMed: 27419376
Data sheet 172_002.pdf

References for Vimentin - 172 002

Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
A Simple DMSO-Based Method for Cryopreservation of Primary Hippocampal and Cortical Neurons.
Ishizuka Y, Bramham CR
Journal of neuroscience methods (2019) : 108578. 172 002 WB; tested species: rat
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 172 002 WB
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment.
Sugita S, Yamato M, Hatabu T, Kataoka Y
Scientific reports (2021) 111: 9668. 172 002 IHC; tested species: mouse
Tanycytes and a differential fatty acid metabolism in the hypothalamus.
Hofmann K, Lamberz C, Piotrowitz K, Offermann N, But D, Scheller A, Al-Amoudi A, Kuerschner L
Glia (2017) 652: 231-249. 172 002 IHC; tested species: mouse
KCa3.1 channels modulate the processing of noxious chemical stimuli in mice.
Lu R, Flauaus C, Kennel L, Petersen J, Drees O, Kallenborn-Gerhardt W, Ruth P, Lukowski R, Schmidtko A
Neuropharmacology (2017) 125: 386-395. 172 002 IHC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified
NDRG2 as a marker protein for brain astrocytes.
Flügge G, Araya-Callis C, Garea-Rodriguez E, Stadelmann-Nessler C, Fuchs E
Cell and tissue research (2014) 3571: 31-41. 172 002 IHC; tested species: marmoset
Age-related brain pathology in Octodon degu: blood vessel, white matter and Alzheimer-like pathology.
van Groen T, Kadish I, Popović N, Popović M, Caballero-Bleda M, Baño-Otálora B, Vivanco P, Rol MÁ, Madrid JA
Neurobiology of aging (2011) 329: 1651-61. 172 002 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 172 002 IHC
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
Cat. No.: 172 002
Amount: 200 µl
Price: $355.00
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
A Simple DMSO-Based Method for Cryopreservation of Primary Hippocampal and Cortical Neurons.
Ishizuka Y, Bramham CR
Journal of neuroscience methods (2019) : 108578. 172 002 WB; tested species: rat
A novel method for culturing stellate astrocytes reveals spatially distinct Ca2+ signaling and vesicle recycling in astrocytic processes.
Wolfes AC, Ahmed S, Awasthi A, Stahlberg MA, Rajput A, Magruder DS, Bonn S, Dean C
The Journal of general physiology (2017) 1491: 149-170. 172 002 WB
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
Involvement of cancer-derived EMT cells in the accumulation of 18F-fluorodeoxyglucose in the hypoxic cancer microenvironment.
Sugita S, Yamato M, Hatabu T, Kataoka Y
Scientific reports (2021) 111: 9668. 172 002 IHC; tested species: mouse
Tanycytes and a differential fatty acid metabolism in the hypothalamus.
Hofmann K, Lamberz C, Piotrowitz K, Offermann N, But D, Scheller A, Al-Amoudi A, Kuerschner L
Glia (2017) 652: 231-249. 172 002 IHC; tested species: mouse
KCa3.1 channels modulate the processing of noxious chemical stimuli in mice.
Lu R, Flauaus C, Kennel L, Petersen J, Drees O, Kallenborn-Gerhardt W, Ruth P, Lukowski R, Schmidtko A
Neuropharmacology (2017) 125: 386-395. 172 002 IHC; tested species: mouse
The intermediate filament protein vimentin is essential for axonotrophic effects of Clostridium botulinum C3 exoenzyme.
Adolf A, Leondaritis G, Rohrbeck A, Eickholt BJ, Just I, Ahnert-Hilger G, Höltje M
Journal of neurochemistry (2016) 1392: 234-244. 172 002 IHC, WB; KO verified
NDRG2 as a marker protein for brain astrocytes.
Flügge G, Araya-Callis C, Garea-Rodriguez E, Stadelmann-Nessler C, Fuchs E
Cell and tissue research (2014) 3571: 31-41. 172 002 IHC; tested species: marmoset
Age-related brain pathology in Octodon degu: blood vessel, white matter and Alzheimer-like pathology.
van Groen T, Kadish I, Popović N, Popović M, Caballero-Bleda M, Baño-Otálora B, Vivanco P, Rol MÁ, Madrid JA
Neurobiology of aging (2011) 329: 1651-61. 172 002 IHC
Activity-dependent regulation of MHC class I expression in the developing primary visual cortex of the common marmoset monkey.
Ribic A, Flügge G, Schlumbohm C, Mätz-Rensing K, Walter L, Fuchs E
Behavioral and brain functions : BBF (2011) 7: 1. 172 002 IHC
Release of astroglial vimentin by extracellular vesicles: Modulation of binding and internalization of C3 transferase in astrocytes and neurons.
Adolf A, Rohrbeck A, Münster-Wandowski A, Johansson M, Kuhn HG, Kopp MA, Brommer B, Schwab JM, Just I, Ahnert-Hilger G, Höltje M, et al.
Glia (2018) : . 172 002 WB, ICC, IHC, EM; KO verified
WB
ICC
IHC
IHC-P
Background
Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.
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