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GFAP antibody - 173 006 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Chicken polyclonal purified antibody
Cat. No.: 173 006
Amount: 50 µg
Price: $390.00
Cat. No. 173 006 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended (see remarks)
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 200 up to 1 : 500 gallery  
DNA-PAINT: 1 : 500
iDISCO: 1 : 400

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

In antibody-based DNA-PAINT (Point Accumulation in Nanoscale Topography), a short oligomeric docking strand is coupled to a specific antibody. The transient association of the fluorophore to the antibody is mediated by the pairing of a short fluorescently labeled complementary imager DNA strand. DNA-PAINT allows super-resolution imaging and the imaging of a huge number of antibodies on the same biological sample in a single multiplex experiment. For additional experimental details refer to the Remarks section.

iDISCO is a fast and cost-effective sample preparation method for clearing, immunolabeling and volume imaging of large biological samples. The clearing is carried out by benzyl-ether treatment. For additional experimental details, please refer to the „Remarks“ section or visit https://idisco.info.
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995).
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms. K.O. validated
Matching control protein/peptide 173-0P
Remarks

WB: Cat. nos. 173 002 or 173 011 are recommended.
Data sheet 173_006.pdf

References for GFAP - 173 006

A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.
Lyu Z, Park J, Kim KM, Jin HJ, Wu H, Rajadas J, Kim DH, Steinberg GK, Lee W
Nature biomedical engineering (2021) 58: 847-863. 173 006 ICC; tested species: human
Brain-gut photobiomodulation restores cognitive alterations in chronically stressed mice through the regulation of Sirt1 and neuroinflammation.
Sancho-Balsells A, Borràs-Pernas S, Flotta F, Chen W, Del Toro D, Rodríguez MJ, Alberch J, Blivet G, Touchon J, Xifró X, Giralt A, et al.
Journal of affective disorders (2024) : . 173 006 IHC; tested species: mouse
Induced Remodelling of Astrocytes In Vitro and In Vivo by Manipulation of Astrocytic RhoA Activity.
Domingos C, Müller FE, Passlick S, Wachten D, Ponimaskin E, Schwarz MK, Schoch S, Zeug A, Henneberger C
Cells (2023) 122: . 173 006 IHC; tested species: mouse
The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 173 006 IHC; tested species: mouse
Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 173 006 IHC; tested species: mouse
Dopamine-induced calcium signaling in olfactory bulb astrocytes.
Fischer T, Scheffler P, Lohr C
Scientific reports (2020) 101: 631. 173 006 IHC; tested species: mouse
Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain.
Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, Farkas E, et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X19900097. 173 006 IHC; tested species: mouse
Amyloid β induces interneuron-specific changes in the hippocampus of APPNL-F mice.
Sos KE, Mayer MI, Takács VT, Major A, Bardóczi Z, Beres BM, Szeles T, Saito T, Saido TC, Mody I, Freund TF, et al.
PloS one (2020) 155: e0233700. 173 006 IHC; tested species: mouse
Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 173 006 IHC; tested species: mouse
Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 173 006 IHC-P; tested species: mouse
Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 173 006 DNA-PAINT; tested species: rat
Cat. No.: 173 006
Amount: 50 µg
Price: $390.00
A neurovascular-unit-on-a-chip for the evaluation of the restorative potential of stem cell therapies for ischaemic stroke.
Lyu Z, Park J, Kim KM, Jin HJ, Wu H, Rajadas J, Kim DH, Steinberg GK, Lee W
Nature biomedical engineering (2021) 58: 847-863. 173 006 ICC; tested species: human
Brain-gut photobiomodulation restores cognitive alterations in chronically stressed mice through the regulation of Sirt1 and neuroinflammation.
Sancho-Balsells A, Borràs-Pernas S, Flotta F, Chen W, Del Toro D, Rodríguez MJ, Alberch J, Blivet G, Touchon J, Xifró X, Giralt A, et al.
Journal of affective disorders (2024) : . 173 006 IHC; tested species: mouse
Induced Remodelling of Astrocytes In Vitro and In Vivo by Manipulation of Astrocytic RhoA Activity.
Domingos C, Müller FE, Passlick S, Wachten D, Ponimaskin E, Schwarz MK, Schoch S, Zeug A, Henneberger C
Cells (2023) 122: . 173 006 IHC; tested species: mouse
The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.
Tóth K, Lénárt N, Berki P, Fekete R, Szabadits E, Pósfai B, Cserép C, Alatshan A, Benkő S, Kiss D, Hübner CA, et al.
PLoS biology (2022) 201: e3001526. 173 006 IHC; tested species: mouse
Microglia modulate blood flow, neurovascular coupling, and hypoperfusion via purinergic actions.
Császár E, Lénárt N, Cserép C, Környei Z, Fekete R, Pósfai B, Balázsfi D, Hangya B, Schwarcz AD, Szabadits E, Szöllősi D, et al.
The Journal of experimental medicine (2022) 2193: . 173 006 IHC; tested species: mouse
Dopamine-induced calcium signaling in olfactory bulb astrocytes.
Fischer T, Scheffler P, Lohr C
Scientific reports (2020) 101: 631. 173 006 IHC; tested species: mouse
Microglia alter the threshold of spreading depolarization and related potassium uptake in the mouse brain.
Varga DP, Menyhárt Á, Pósfai B, Császár E, Lénárt N, Cserép C, Orsolits B, Martinecz B, Szlepák T, Bari F, Farkas E, et al.
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism (2020) : 271678X19900097. 173 006 IHC; tested species: mouse
Amyloid β induces interneuron-specific changes in the hippocampus of APPNL-F mice.
Sos KE, Mayer MI, Takács VT, Major A, Bardóczi Z, Beres BM, Szeles T, Saito T, Saido TC, Mody I, Freund TF, et al.
PloS one (2020) 155: e0233700. 173 006 IHC; tested species: mouse
Microglia monitor and protect neuronal function through specialized somatic purinergic junctions.
Cserép C, Pósfai B, Lénárt N, Fekete R, László ZI, Lele Z, Orsolits B, Molnár G, Heindl S, Schwarcz AD, Ujvári K, et al.
Science (New York, N.Y.) (2020) 3676477: 528-537. 173 006 IHC; tested species: mouse
Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 173 006 IHC-P; tested species: mouse
Fast DNA-PAINT imaging using a deep neural network.
Narayanasamy KK, Rahm JV, Tourani S, Heilemann M
Nature communications (2022) 131: 5047. 173 006 DNA-PAINT; tested species: rat
ICC
IHC
IHC
IHC-P
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.
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