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GFAP antibody - 173 011BT K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Mouse monoclonal purified IgG
Cat. No.: 173 011BT
Amount: 100 µg
Price: $470.00
Cat. No. 173 011BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 200 gallery  
Label biotin
Clone 134B1
Subtype IgG2a (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1 (alpha). K.O. validated
Matching control protein/peptide 173-0P
Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
Data sheet 173_011bt.pdf

References for GFAP - 173 011BT

Chronic lithium administration in a mouse model for Krabbe disease.
Del Grosso A, Parlanti G, Angella L, Giordano N, Tonazzini I, Ottalagana E, Carpi S, Pellegrino RM, Alabed HBR, Emiliani C, Caleo M, et al.
JIMD reports (2022) 631: 50-65. 173 011BT WB; tested species: mouse
Cat. No.: 173 011BT
Amount: 100 µg
Price: $470.00
Chronic lithium administration in a mouse model for Krabbe disease.
Del Grosso A, Parlanti G, Angella L, Giordano N, Tonazzini I, Ottalagana E, Carpi S, Pellegrino RM, Alabed HBR, Emiliani C, Caleo M, et al.
JIMD reports (2022) 631: 50-65. 173 011BT WB; tested species: mouse
WB
ICC
IHC
IHC-P
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.
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