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GFAP antibody - 173 211BT K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Mouse monoclonal purified IgG
Cat. No.: 173 211BT
Amount: 100 µg
Price: $465.00
Cat. No. 173 211BT 100 µg purified IgG, lyophilized, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: yes
ICC: not recommended
IHC: not recommended
IHC-P: not tested yet
ELISA: yes (see remarks)
Label biotin
Clone 186C6
Subtype IgG1 (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 1 to 15 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
Other species not tested yet.
Specificity Specific for GFAP, detects all isoforms. K.O. validated
Matching control protein/peptide 173-0P
Remarks

WB: The monoclonal antibodies are less sensitive compared to the rabbit polyclonal polyclonal (cat. no. 173 002).
ELISA: Suitable as detector antibody for sandwich-ELISA with cat. no. 173 011 as capture antibody.
Data sheet 173_211bt.pdf

References for GFAP - 173 211BT

O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies.
Permanne B, Sand A, Ousson S, Nény M, Hantson J, Schubert R, Wiessner C, Quattropani A, Beher D
ACS chemical neuroscience (2022) : . 173 211BT ELISA; tested species: mouse
A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates.
Ganau M, Bosco A, Palma A, Corvaglia S, Parisse P, Fruk L, Beltrami AP, Cesselli D, Casalis L, Scoles G
Nanomedicine : nanotechnology, biology, and medicine (2015) 112: 293-300. 173 211BT ELISA
Cat. No.: 173 211BT
Amount: 100 µg
Price: $465.00
O-GlcNAcase Inhibitor ASN90 is a Multimodal Drug Candidate for Tau and α-Synuclein Proteinopathies.
Permanne B, Sand A, Ousson S, Nény M, Hantson J, Schubert R, Wiessner C, Quattropani A, Beher D
ACS chemical neuroscience (2022) : . 173 211BT ELISA; tested species: mouse
A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates.
Ganau M, Bosco A, Palma A, Corvaglia S, Parisse P, Fruk L, Beltrami AP, Cesselli D, Casalis L, Scoles G
Nanomedicine : nanotechnology, biology, and medicine (2015) 112: 293-300. 173 211BT ELISA
WB
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.
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