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GFAP antibody - 173 308 K.O.

GFAP is an astrocyte-specific type-III intermediate filament protein
Guinea pig monoclonal recombinant IgG
Cat. No.: 173 308
Amount: 50 µg
Price: $415.00
Cat. No. 173 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) (see remarks) gallery  
IP: not tested yet
ICC: 1 : 1000 gallery  
IHC: 1 : 2000 gallery  
IHC-P: 1 : 2000 up to 1 : 5000 gallery  
IHC-Fr: 1 : 2000 (see remarks) gallery  
Clone Gp134B1
Subtype IgG2 (κ light chain)
Immunogen full-length recombinant human GFAP (UniProt Id: P14136)
Epitop AA 391 to 405 from human GFAP (UniProt Id: P14136)
Reactivity Reacts with: human (P14136), rat (P47819), mouse (P03995), cow.
No signal: zebrafish.
Other species not tested yet.
Specificity Specific for GFAP isoform 1 K.O. validated
Matching control protein/peptide 173-0P
Remarks

This antibody is a chimeric antibody based on the well known monoclonal mouse antibody clone 134B1. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
WB: In Western blots, the monoclonal GFAP antibodies are less sensitive than the polyclonal rabbit and Guinea pig GFAP antibodies.
IHC-Fr: Fixation with acetone, methanol, acetone-methanol or PFA/formaldehyde is recommended. Signal intensities as follows: PFA = acetone > acetone-methanol = methanol.
Data sheet 173_308.pdf

References for GFAP - 173 308

Lifelong absence of microglia alters hippocampal glutamatergic networks but not synapse and spine density.
Surala M, Soso-Zdravkovic L, Munro D, Rifat A, Ouk K, Vida I, Priller J, Madry C
EMBO reports (2024) : . 173 308 IHC; tested species: mouse
Cat. No.: 173 308
Amount: 50 µg
Price: $415.00
Lifelong absence of microglia alters hippocampal glutamatergic networks but not synapse and spine density.
Surala M, Soso-Zdravkovic L, Munro D, Rifat A, Ouk K, Vida I, Priller J, Madry C
EMBO reports (2024) : . 173 308 IHC; tested species: mouse
WB
ICC
IHC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-Fr
IHC-Fr
Background

Glial fibrillary acidic protein GFAP is a glial-specific member of the intermediate filament protein family. This group comprises cell type-specific filamentous proteins with similar structure and function as scaffold for cytoskeleton assembly and maintenance.
Frequently, neural stem cells also express GFAP. In addition many types of brain tumors, probably derived from astrocytic cells, heavily express GFAP. This protein is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and others.
Point-mutations in the GFAP gene have been correlated to Alexander disease, a fatal leukoencephalopathy that leads to the dysmyelination or demyelination of the central nervous system.
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