IBA1 antibody - 234 009

IBA1 is a marker for macrophages and microglia
Chicken monoclonal recombinant IgY
Cat. No.: 234 009
Amount: 50 µg
Price: $425.00
Cat. No. 234 009 50 µg purified recombinant IgY, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 200 up to 1 : 1000 gallery  
ExM: 1 : 100

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Expansion Microscopy (ExM) is a sample preparation and imaging method which employs a dense interconnected web of swellable polymer within a biological specimen. A significantly higher effective resolution can be achieved with a standard microscope setup. For additional experimental details, please refer to the Remarks section.

Clone Ch311H9
Subtype IgY (λ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: rat (P55009), mouse (Q9EQW9), human (P55008), ape.
Other species not tested yet.
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by chicken specific sequences. Therefore, the antibody can be used with standard anti-chicken secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet 234_009.pdf

References for IBA1 - 234 009

Primary cortical cell tri-culture to study effects of amyloid-β on microglia function and neuroinflammatory response.
Kim H, Le B, Goshi N, Zhu K, Grodzki AC, Lein PJ, Zhao M, Seker E
Journal of Alzheimer's disease : JAD (2024) : 13872877241291142. 234 009 ICC; tested species: rat
Increased inflammation in older high-pressure glaucoma mice.
Reinehr S, Rahim Pamuk M, Fuchshofer R, Burkhard Dick H, Joachim SC
Neurobiology of aging (2024) 145: 55-64. 234 009 IHC; tested species: mouse
Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model.
Deppe L, Mueller-Buehl AM, Tsai T, Erb C, Dick HB, Joachim SC
Journal of personalized medicine (2024) 144: . 234 009 IHC; tested species: pig
Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation.
Tegowski M, Prater AK, Holley CL, Meyer KD
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse
Chronic glial activation and behavioral alterations induced by acute/subacute pioglitazone treatment in a mouse model of traumatic brain injury.
Estrella LD, Manganaro JE, Sheldon L, Roland N, Snyder AD, George JW, Emanuel K, Lamberty BG, Stauch KL
Brain, behavior, and immunity (2024) 123: 64-80. 234 009 IHC; tested species: mouse
Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 009 IHC; tested species: mouse
Microglia regulate sleep through calcium-dependent modulation of norepinephrine transmission.
Ma C, Li B, Silverman D, Ding X, Li A, Xiao C, Huang G, Worden K, Muroy S, Chen W, Xu Z, et al.
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse
Microglia maintain structural integrity during fetal brain morphogenesis.
Lawrence AR, Canzi A, Bridlance C, Olivié N, Lansonneur C, Catale C, Pizzamiglio L, Kloeckner B, Silvin A, Munro DAD, Fortoul A, et al.
Cell (2024) : . 234 009 IHC; tested species: mouse
Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury.
Androvic P, Schifferer M, Perez Anderson K, Cantuti-Castelvetri L, Jiang H, Ji H, Liu L, Gouna G, Berghoff SA, Besson-Girard S, Knoferle J, et al.
Nature communications (2023) 141: 4115. 234 009 IHC; tested species: mouse
Control of hippocampal synaptic plasticity by microglia-dendrite interactions depends on genetic context in mouse models of Alzheimer's disease.
Heuer SE, Keezer KJ, Hewes AA, Onos KD, Graham KC, Howell GR, Bloss EB
Alzheimer's & dementia : the journal of the Alzheimer's Association (2023) : . 234 009 IHC; tested species: mouse
HSP27 induced glaucomatous damage in mice of young and advanced age.
Erb C, Reinehr S, Theiss C, Dick HB, Joachim SC
Frontiers in cellular neuroscience (2023) 17: 1257297. 234 009 IHC; tested species: mouse
Activation of β2-Adrenergic Receptors in Microglia Alleviates Neuropathic Hypersensitivity in Mice.
Damo E, Agarwal A, Simonetti M
Cells (2023) 122: . 234 009 IHC; tested species: mouse
Lipofuscin-like autofluorescence within microglia and its impact on studying microglial engulfment.
Stillman JM, Mendes Lopes F, Lin JP, Hu K, Reich DS, Schafer DP
Nature communications (2023) 141: 7060. 234 009 IHC; tested species: mouse
Meningeal macrophages protect against viral neuroinfection.
Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, Bardin Q, et al.
Immunity (2022) 5511: 2103-2117.e10. 234 009 IHC; tested species: monkey
Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring.
Smith CJ, Lintz T, Clark MJ, Malacon KE, Abiad A, Constantino NJ, Kim VJ, Jo YC, Alonso-Caraballo Y, Bilbo SD, Chartoff EH, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) 4710: 1755-1763. 234 009 IHC; tested species: rat
Activation of GPR183 by 7α,25-Dihydroxycholesterol Induces Behavioral Hypersensitivity through Mitogen-Activated Protein Kinase and Nuclear Factor-κB.
Braden K, Campolo M, Li Y, Chen Z, Doyle TM, Giancotti LA, Esposito E, Zhang J, Cuzzocrea S, Arnatt CK, Salvemini D, et al.
The Journal of pharmacology and experimental therapeutics (2022) 3832: 172-181. 234 009 IHC; tested species: mouse
The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 234 009 IHC; tested species: mouse
Glutamate Metabotropic Receptor Type 3 (mGlu3) Localization in the Rat Prelimbic Medial Prefrontal Cortex.
Woo E, Datta D, Arnsten AFT
Frontiers in neuroanatomy (2022) 16: 849937. 234 009 IHC; tested species: rat
Analgesic candidate adenosine A 3 receptors are expressed by perineuronal peripheral macrophages in human dorsal root ganglion and spinal cord microglia.
Sapio MR, Staedtler ES, King DM, Maric D, Jahanipour J, Ghetti A, Jacobson KA, Mannes AJ, Iadarola MJ
Pain (2024) 16510: 2323-2343. 234 009 IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 IHC-P, ExM; tested species: human
Single-cell image analysis reveals over-expression of organic anion transporting polypeptides (OATPs) in human glioblastoma tissue.
Cooper E, Woolf Z, Swanson MEV, Correia J, Schweder P, Mee E, Heppner P, Turner C, Faull RLM, Scotter EL, Denny WA, et al.
Neuro-oncology advances (2022) 41: vdac166. 234 009 IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 IHC-P, ExM; tested species: human
Cat. No.: 234 009
Amount: 50 µg
Price: $425.00
Primary cortical cell tri-culture to study effects of amyloid-β on microglia function and neuroinflammatory response.
Kim H, Le B, Goshi N, Zhu K, Grodzki AC, Lein PJ, Zhao M, Seker E
Journal of Alzheimer's disease : JAD (2024) : 13872877241291142. 234 009 ICC; tested species: rat
Increased inflammation in older high-pressure glaucoma mice.
Reinehr S, Rahim Pamuk M, Fuchshofer R, Burkhard Dick H, Joachim SC
Neurobiology of aging (2024) 145: 55-64. 234 009 IHC; tested species: mouse
Protection against Oxidative Stress by Coenzyme Q10 in a Porcine Retinal Degeneration Model.
Deppe L, Mueller-Buehl AM, Tsai T, Erb C, Dick HB, Joachim SC
Journal of personalized medicine (2024) 144: . 234 009 IHC; tested species: pig
Single-cell m6A profiling in the mouse brain uncovers cell type-specific RNA methylomes and age-dependent differential methylation.
Tegowski M, Prater AK, Holley CL, Meyer KD
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse
Chronic glial activation and behavioral alterations induced by acute/subacute pioglitazone treatment in a mouse model of traumatic brain injury.
Estrella LD, Manganaro JE, Sheldon L, Roland N, Snyder AD, George JW, Emanuel K, Lamberty BG, Stauch KL
Brain, behavior, and immunity (2024) 123: 64-80. 234 009 IHC; tested species: mouse
Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 009 IHC; tested species: mouse
Microglia regulate sleep through calcium-dependent modulation of norepinephrine transmission.
Ma C, Li B, Silverman D, Ding X, Li A, Xiao C, Huang G, Worden K, Muroy S, Chen W, Xu Z, et al.
Nature neuroscience (2024) : . 234 009 IHC; tested species: mouse
Microglia maintain structural integrity during fetal brain morphogenesis.
Lawrence AR, Canzi A, Bridlance C, Olivié N, Lansonneur C, Catale C, Pizzamiglio L, Kloeckner B, Silvin A, Munro DAD, Fortoul A, et al.
Cell (2024) : . 234 009 IHC; tested species: mouse
Spatial Transcriptomics-correlated Electron Microscopy maps transcriptional and ultrastructural responses to brain injury.
Androvic P, Schifferer M, Perez Anderson K, Cantuti-Castelvetri L, Jiang H, Ji H, Liu L, Gouna G, Berghoff SA, Besson-Girard S, Knoferle J, et al.
Nature communications (2023) 141: 4115. 234 009 IHC; tested species: mouse
Control of hippocampal synaptic plasticity by microglia-dendrite interactions depends on genetic context in mouse models of Alzheimer's disease.
Heuer SE, Keezer KJ, Hewes AA, Onos KD, Graham KC, Howell GR, Bloss EB
Alzheimer's & dementia : the journal of the Alzheimer's Association (2023) : . 234 009 IHC; tested species: mouse
HSP27 induced glaucomatous damage in mice of young and advanced age.
Erb C, Reinehr S, Theiss C, Dick HB, Joachim SC
Frontiers in cellular neuroscience (2023) 17: 1257297. 234 009 IHC; tested species: mouse
Activation of β2-Adrenergic Receptors in Microglia Alleviates Neuropathic Hypersensitivity in Mice.
Damo E, Agarwal A, Simonetti M
Cells (2023) 122: . 234 009 IHC; tested species: mouse
Lipofuscin-like autofluorescence within microglia and its impact on studying microglial engulfment.
Stillman JM, Mendes Lopes F, Lin JP, Hu K, Reich DS, Schafer DP
Nature communications (2023) 141: 7060. 234 009 IHC; tested species: mouse
Meningeal macrophages protect against viral neuroinfection.
Rebejac J, Eme-Scolan E, Arnaud Paroutaud L, Kharbouche S, Teleman M, Spinelli L, Gallo E, Roussel-Queval A, Zarubica A, Sansoni A, Bardin Q, et al.
Immunity (2022) 5511: 2103-2117.e10. 234 009 IHC; tested species: monkey
Prenatal opioid exposure inhibits microglial sculpting of the dopamine system selectively in adolescent male offspring.
Smith CJ, Lintz T, Clark MJ, Malacon KE, Abiad A, Constantino NJ, Kim VJ, Jo YC, Alonso-Caraballo Y, Bilbo SD, Chartoff EH, et al.
Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology (2022) 4710: 1755-1763. 234 009 IHC; tested species: rat
Activation of GPR183 by 7α,25-Dihydroxycholesterol Induces Behavioral Hypersensitivity through Mitogen-Activated Protein Kinase and Nuclear Factor-κB.
Braden K, Campolo M, Li Y, Chen Z, Doyle TM, Giancotti LA, Esposito E, Zhang J, Cuzzocrea S, Arnatt CK, Salvemini D, et al.
The Journal of pharmacology and experimental therapeutics (2022) 3832: 172-181. 234 009 IHC; tested species: mouse
The RNA-Binding Protein HuR Is Integral to the Function of Nociceptors in Mice and Humans.
Kunder N, de la Peña JB, Lou TF, Chase R, Suresh P, Lawson J, Shukla T, Black B, Campbell ZT
The Journal of neuroscience : the official journal of the Society for Neuroscience (2022) 4249: 9129-9141. 234 009 IHC; tested species: mouse
Glutamate Metabotropic Receptor Type 3 (mGlu3) Localization in the Rat Prelimbic Medial Prefrontal Cortex.
Woo E, Datta D, Arnsten AFT
Frontiers in neuroanatomy (2022) 16: 849937. 234 009 IHC; tested species: rat
Analgesic candidate adenosine A 3 receptors are expressed by perineuronal peripheral macrophages in human dorsal root ganglion and spinal cord microglia.
Sapio MR, Staedtler ES, King DM, Maric D, Jahanipour J, Ghetti A, Jacobson KA, Mannes AJ, Iadarola MJ
Pain (2024) 16510: 2323-2343. 234 009 IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 IHC-P, ExM; tested species: human
Single-cell image analysis reveals over-expression of organic anion transporting polypeptides (OATPs) in human glioblastoma tissue.
Cooper E, Woolf Z, Swanson MEV, Correia J, Schweder P, Mee E, Heppner P, Turner C, Faull RLM, Scotter EL, Denny WA, et al.
Neuro-oncology advances (2022) 41: vdac166. 234 009 IHC-P; tested species: human
Changes in glial cell phenotypes precede overt neurofibrillary tangle formation, correlate with markers of cortical cell damage, and predict cognitive status of individuals at Braak III-IV stages.
Taddei RN, Sanchez-Mico MV, Bonnar O, Connors T, Gaona A, Denbow D, Frosch MP, Gómez-Isla T
Acta neuropathologica communications (2022) 101: 72. 234 009 IHC-P, ExM; tested species: human
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).