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IBA1 antibody - 234 308

IBA1 is a marker for macrophages and microglia
Guinea pig monoclonal recombinant IgG
Cat. No.: 234 308
Amount: 50 µg
Price: $425.00
Cat. No. 234 308 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: yes
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
Clone Gp311H9
Subtype IgG2 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of rat IBA1 (UniProt Id: P55009)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008), monkey.
Other species not tested yet.
Matching control protein/peptide 234-0P
Remarks

This antibody is a chimeric antibody based on the monoclonal mouse antibody clone 311H9. The constant regions of the heavy and light chains have been replaced by Guinea pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet 234_308.pdf

References for IBA1 - 234 308

Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization.
Wang N, Li F, Du J, Hao J, Wang X, Hou Y, Luo Z
Journal of inflammation research (2024) 17: 1281-1293. 234 308 WB, IHC; tested species: rat
Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.
Park JC, Han JW, Lee W, Kim J, Lee SE, Lee D, Choi H, Han J, Kang YJ, Diep YN, Cho H, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400064. 234 308 ICC, IHC; tested species: human,mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 234 308 ICC, IHC; tested species: mouse
Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 234 308 ICC; tested species: mouse
Retinal response to systemic inflammation differs between sexes and neurons.
Rodríguez-Ramírez KT, Norte-Muñoz M, Lucas-Ruiz F, Gallego-Ortega A, Calzaferri F, García-Bernal D, Martínez CM, Galindo-Romero C, de Los Ríos C, Vidal-Sanz M, Agudo-Barriuso M, et al.
Frontiers in immunology (2024) 15: 1340013. 234 308 IHC; tested species: mouse
Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization.
Wang N, Li F, Du J, Hao J, Wang X, Hou Y, Luo Z
Journal of inflammation research (2024) 17: 1281-1293. 234 308 WB, IHC; tested species: rat
Nigrostriatal degeneration determines dynamics of glial inflammatory and phagocytic activity.
Ayerra L, Abellanas MA, Basurco L, Tamayo I, Conde E, Tavira A, Trigo A, Vidaurre C, Vilas A, San Martin-Uriz P, Luquin E, et al.
Journal of neuroinflammation (2024) 211: 92. 234 308 IHC; tested species: mouse
Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.
Park JC, Han JW, Lee W, Kim J, Lee SE, Lee D, Choi H, Han J, Kang YJ, Diep YN, Cho H, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400064. 234 308 ICC, IHC; tested species: human,mouse
IRF8 and MAFB drive distinct transcriptional machineries in different resident macrophages of the central nervous system.
Yamasaki A, Imanishi I, Tanaka K, Ohkawa Y, Tsuda M, Masuda T
Communications biology (2024) 71: 896. 234 308 IHC; tested species: mouse
Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 308 IHC; tested species: mouse
hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.
Zare A, Salehi S, Bader J, Schneider C, Fischer U, Veh A, Arampatzi P, Mann M, Briese M, Sendtner M
Nature communications (2024) 151: 7430. 234 308 IHC; tested species: mouse
Fibrin drives thromboinflammation and neuropathology in COVID-19.
Ryu JK, Yan Z, Montano M, Sozmen EG, Dixit K, Suryawanshi RK, Matsui Y, Helmy E, Kaushal P, Makanani SK, Deerinck TJ, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse
Myocardial infarction augments sleep to limit cardiac inflammation and damage.
Huynh P, Hoffmann JD, Gerhardt T, Kiss MG, Zuraikat FM, Cohen O, Wolfram C, Yates AG, Leunig A, Heiser M, Gaebel L, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse
Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19.
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, et al.
Cell host & microbe (2024) : . 234 308 IHC; tested species: human,mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 234 308 ICC, IHC; tested species: mouse
Receptor-interacting protein kinase 2 (RIPK2) profoundly contributes to post-stroke neuroinflammation and behavioral deficits with microglia as unique perpetrators.
Larochelle J, Tishko RJ, Yang C, Ge Y, Phan LT, Gunraj RE, Stansbury SM, Liu L, Mohamadzadeh M, Khoshbouei H, Candelario-Jalil E, et al.
Journal of neuroinflammation (2023) 201: 221. 234 308 IHC; tested species: mouse
Trem2 expression in microglia is required to maintain normal neuronal bioenergetics during development.
Tagliatti E, Desiato G, Mancinelli S, Bizzotto M, Gagliani MC, Faggiani E, Hernández-Soto R, Cugurra A, Poliseno P, Miotto M, Argüello RJ, et al.
Immunity (2023) : . 234 308 IHC; tested species: mouse
Microglial Nogo delays recovery following traumatic brain injury in mice.
Glotfelty EJ, Hsueh SC, Claybourne Q, Bedolla A, Kopp KO, Wallace T, Zheng B, Luo Y, Karlsson TE, McDevitt RA, Olson L, et al.
Glia (2023) 7110: 2473-2494. 234 308 IHC; tested species: mouse
Human microglial state dynamics in Alzheimer's disease progression.
Sun N, Victor MB, Park YP, Xiong X, Scannail AN, Leary N, Prosper S, Viswanathan S, Luna X, Boix CA, James BT, et al.
Cell (2023) 18620: 4386-4403.e29. 234 308 IHC; tested species: human
Reliable detection of RNA in hippocampus sections of mice by FISH up to a post-mortem delay of 24 h.
Seiffer S, Brendler J, Schulz A, Ricken A
Histochemistry and cell biology (2024) : . 234 308 IHC-P; tested species: mouse
Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 234 308 IHC-P; tested species: mouse
Cat. No.: 234 308
Amount: 50 µg
Price: $425.00
Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization.
Wang N, Li F, Du J, Hao J, Wang X, Hou Y, Luo Z
Journal of inflammation research (2024) 17: 1281-1293. 234 308 WB, IHC; tested species: rat
Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.
Park JC, Han JW, Lee W, Kim J, Lee SE, Lee D, Choi H, Han J, Kang YJ, Diep YN, Cho H, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400064. 234 308 ICC, IHC; tested species: human,mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 234 308 ICC, IHC; tested species: mouse
Microglia modulate TNFα-mediated synaptic plasticity.
Kleidonas D, Kirsch M, Andrieux G, Pfeifer D, Boerries M, Vlachos A
Glia (2023) 719: 2117-2136. 234 308 ICC; tested species: mouse
Retinal response to systemic inflammation differs between sexes and neurons.
Rodríguez-Ramírez KT, Norte-Muñoz M, Lucas-Ruiz F, Gallego-Ortega A, Calzaferri F, García-Bernal D, Martínez CM, Galindo-Romero C, de Los Ríos C, Vidal-Sanz M, Agudo-Barriuso M, et al.
Frontiers in immunology (2024) 15: 1340013. 234 308 IHC; tested species: mouse
Quercetin Protects Against Global Cerebral ischemia‒reperfusion Injury by Inhibiting Microglial Activation and Polarization.
Wang N, Li F, Du J, Hao J, Wang X, Hou Y, Luo Z
Journal of inflammation research (2024) 17: 1281-1293. 234 308 WB, IHC; tested species: rat
Nigrostriatal degeneration determines dynamics of glial inflammatory and phagocytic activity.
Ayerra L, Abellanas MA, Basurco L, Tamayo I, Conde E, Tavira A, Trigo A, Vidaurre C, Vilas A, San Martin-Uriz P, Luquin E, et al.
Journal of neuroinflammation (2024) 211: 92. 234 308 IHC; tested species: mouse
Microglia Gravitate toward Amyloid Plaques Surrounded by Externalized Phosphatidylserine via TREM2.
Park JC, Han JW, Lee W, Kim J, Lee SE, Lee D, Choi H, Han J, Kang YJ, Diep YN, Cho H, et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany) (2024) : e2400064. 234 308 ICC, IHC; tested species: human,mouse
IRF8 and MAFB drive distinct transcriptional machineries in different resident macrophages of the central nervous system.
Yamasaki A, Imanishi I, Tanaka K, Ohkawa Y, Tsuda M, Masuda T
Communications biology (2024) 71: 896. 234 308 IHC; tested species: mouse
Innate immune training restores pro-reparative myeloid functions to promote remyelination in the aged central nervous system.
Tiwari V, Prajapati B, Asare Y, Damkou A, Ji H, Liu L, Naser N, Gouna G, Leszczyńska KB, Mieczkowski J, Dichgans M, et al.
Immunity (2024) : . 234 308 IHC; tested species: mouse
hnRNP R promotes O-GlcNAcylation of eIF4G and facilitates axonal protein synthesis.
Zare A, Salehi S, Bader J, Schneider C, Fischer U, Veh A, Arampatzi P, Mann M, Briese M, Sendtner M
Nature communications (2024) 151: 7430. 234 308 IHC; tested species: mouse
Fibrin drives thromboinflammation and neuropathology in COVID-19.
Ryu JK, Yan Z, Montano M, Sozmen EG, Dixit K, Suryawanshi RK, Matsui Y, Helmy E, Kaushal P, Makanani SK, Deerinck TJ, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse
Myocardial infarction augments sleep to limit cardiac inflammation and damage.
Huynh P, Hoffmann JD, Gerhardt T, Kiss MG, Zuraikat FM, Cohen O, Wolfram C, Yates AG, Leunig A, Heiser M, Gaebel L, et al.
Nature (2024) : . 234 308 IHC; tested species: mouse
Persistence of spike protein at the skull-meninges-brain axis may contribute to the neurological sequelae of COVID-19.
Rong Z, Mai H, Ebert G, Kapoor S, Puelles VG, Czogalla J, Hu S, Su J, Prtvar D, Singh I, Schädler J, et al.
Cell host & microbe (2024) : . 234 308 IHC; tested species: human,mouse
The RhoA-ROCK1/ROCK2 Pathway Exacerbates Inflammatory Signaling in Immortalized and Primary Microglia.
Glotfelty EJ, Tovar-Y-Romo LB, Hsueh SC, Tweedie D, Li Y, Harvey BK, Hoffer BJ, Karlsson TE, Olson L, Greig NH
Cells (2023) 1210: . 234 308 ICC, IHC; tested species: mouse
Receptor-interacting protein kinase 2 (RIPK2) profoundly contributes to post-stroke neuroinflammation and behavioral deficits with microglia as unique perpetrators.
Larochelle J, Tishko RJ, Yang C, Ge Y, Phan LT, Gunraj RE, Stansbury SM, Liu L, Mohamadzadeh M, Khoshbouei H, Candelario-Jalil E, et al.
Journal of neuroinflammation (2023) 201: 221. 234 308 IHC; tested species: mouse
Trem2 expression in microglia is required to maintain normal neuronal bioenergetics during development.
Tagliatti E, Desiato G, Mancinelli S, Bizzotto M, Gagliani MC, Faggiani E, Hernández-Soto R, Cugurra A, Poliseno P, Miotto M, Argüello RJ, et al.
Immunity (2023) : . 234 308 IHC; tested species: mouse
Microglial Nogo delays recovery following traumatic brain injury in mice.
Glotfelty EJ, Hsueh SC, Claybourne Q, Bedolla A, Kopp KO, Wallace T, Zheng B, Luo Y, Karlsson TE, McDevitt RA, Olson L, et al.
Glia (2023) 7110: 2473-2494. 234 308 IHC; tested species: mouse
Human microglial state dynamics in Alzheimer's disease progression.
Sun N, Victor MB, Park YP, Xiong X, Scannail AN, Leary N, Prosper S, Viswanathan S, Luna X, Boix CA, James BT, et al.
Cell (2023) 18620: 4386-4403.e29. 234 308 IHC; tested species: human
Reliable detection of RNA in hippocampus sections of mice by FISH up to a post-mortem delay of 24 h.
Seiffer S, Brendler J, Schulz A, Ricken A
Histochemistry and cell biology (2024) : . 234 308 IHC-P; tested species: mouse
Targeting the glycine-rich domain of TDP-43 with antibodies prevents its aggregation in vitro and reduces neurofilament levels in vivo.
Riemenschneider H, Simonetti F, Sheth U, Katona E, Roth S, Hutten S, Farny D, Michaelsen M, Nuscher B, Schmidt MK, Flatley A, et al.
Acta neuropathologica communications (2023) 111: 112. 234 308 IHC-P; tested species: mouse
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Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3). 
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