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Glutamine synthetase antibody - 367 004

GS is an enzyme for glutamine synthesis and ammonia detoxification, a marker for astrocytes
Guinea pig polyclonal antiserum
Cat. No.: 367 004
Amount: 100 µl
Price: $370.00
Cat. No. 367 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: not tested yet

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Full-length recombinant mouse Glutamine synthetase (UniProt Id: P15105)
Reactivity Reacts with: rat (P09606), mouse (P15105).
Other species not tested yet.
Remarks

IHC: For minimal background, the affinity purified antibody cat. no. 367 005 is recommended
Data sheet 367_004.pdf
Cat. No.: 367 004
Amount: 100 µl
Price: $370.00
WB
IHC
Background
Glutamine synthetase, also referred to as Glutamate-ammonia ligase or GS, is an enzyme that catalyzes the ATP-dependent condensation of glutamate with ammonia to yield glutamine. It is present predominantly in brain, kidneys, and liver. In the brain, it is particularly found in astrocytes.
Glutamine synthetase plays a pivotal role in glutamate and glutamine homoeostasis, and it is largely responsible for the removal of both blood-derived and metabolically generated ammonia, preventing neurotoxicity. It is also a key enzyme in the recycling of the neurotransmitter glutamate.
Several studies indicated that the expression, distribution, and activity of brain glutamine synthetase is altered in some brain disorders, including Alzheimer's disease, schizophrenia, depression, suicidality, and mesial temporal lobe epilepsy (MTLE).
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