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Chil3 (YM1) antibody - 442 004

Chil3/YM1 is a marker protein for murine neutrophils and M2 macrophages
Guinea pig polyclonal antiserum
Cat. No.: 442 004
Amount: 100 µl
Price: $370.00
Cat. No. 442 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 1000 up to 1 : 2000 gallery  
IHC-P: 1 : 1000 up to 1 : 2000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 275 to 297 from mouse Chil3 (UniProt Id: O35744)
Reactivity Reacts with: mouse (O35744).
Other species not tested yet.
Specificity Specific for murine Chil3 / YM1, shows a cross-reactivity with Chil4 (Ym2)
Matching control protein/peptide 442-0P
Data sheet 442_004.pdf

References for Chil3 - 442 004

Abnormal brain structure and behavior in MyD88-deficient mice.
Schroeder P, Rivalan M, Zaqout S, Krüger C, Schüler J, Long M, Meisel A, Winter Y, Kaindl AM, Lehnardt S
Brain, behavior, and immunity (2020) : . 442 004 IHC; tested species: mouse
Cat. No.: 442 004
Amount: 100 µl
Price: $370.00
Abnormal brain structure and behavior in MyD88-deficient mice.
Schroeder P, Rivalan M, Zaqout S, Krüger C, Schüler J, Long M, Meisel A, Winter Y, Kaindl AM, Lehnardt S
Brain, behavior, and immunity (2020) : . 442 004 IHC; tested species: mouse
WB
WB
ICC
IHC
IHC
IHC
IHC-P
IHC-P
Background

Chitinase-like protein 3 (Chil3), commonly referred to as YM1, is a rodent-specific secreted glycoprotein that belongs to the chitinase-like protein (CLP) family, sharing structural similarity with true chitinases but lacking enzymatic activity. It is predominantly expressed by alternatively activated (M2) macrophages, eosinophils, and neutrophils in mice, particularly under type 2 immune conditions such as allergic inflammation, parasitic infection, and tissue repair (1). Chil3 / YM1 expression is strongly induced by IL-4 and IL-13, hallmark cytokines of Th2-driven responses. Alongside Arginase-1 (Arg1) and Fizz1 (Retnla), Chil3 / YM1 is commonly used as a biomarker of M2 macrophage polarization in murine models (2). In healthy adult mice, Chil3 / YM1 is constitutively expressed in alveolar macrophages and neutrophils in the lung and in immature neutrophils in the spleen and bone marrow (1). Its isotype Chil4 / YM2 shows a different expression pattern and is mainly expressed in the stomach (3). In the central nervous system microglia can also induce Chil3 / YM1 expression under specific inflammatory conditions, e.g. after stroke (4).
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