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Aldolase C antibody - 464 004

Aldolase C a brain type isozyme of a glycolysis enzyme
Guinea pig polyclonal antiserum
Cat. No.: 464 004
Amount: 100 µl
Price: $370.00
Cat. No. 464 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 5000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 up to 1 : 1000 (see remarks) gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: not recommended

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Recombinant protein corresponding to AA 1 to 364 from human ALDOC (UniProt Id: P09972)
Reactivity Reacts with: mouse (P05063), rat (P09117).
Other species not tested yet.
Specificity Specific for Aldolase C, no cross-reactivity to Aldolase A and B.
Remarks

ICC: Methanol fixation is recommended.
Data sheet 464_004.pdf

References for Aldolase C - 464 004

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 464 004 IHC; tested species: mouse
Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity.
Voerman S, Urbanus BHA, Schonewille M, White JJ, De Zeeuw CI
Communications biology (2022) 51: 1328. 464 004 IHC; tested species: mouse
Cat. No.: 464 004
Amount: 100 µl
Price: $370.00
INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. 464 004 IHC; tested species: mouse
Postsynaptic plasticity of Purkinje cells in mice is determined by molecular identity.
Voerman S, Urbanus BHA, Schonewille M, White JJ, De Zeeuw CI
Communications biology (2022) 51: 1328. 464 004 IHC; tested species: mouse
WB
WB
ICC
ICC
IHC
IHC
IHC
IHC
IHC
IHC
Background

Fructose 1, 6-bisphosphate aldolase catalyses the reversible condensation of glycerone-P and glyceraldehyde 3-phosphate into fructose 1, 6-bisphosphate. Fructose 1, 6-bisphosphate aldolase exists as three forms, the muscle-specific Aldolase A, the liver-specific aldolase B, and the brain-specific aldolase C. (1)
Aldolase C-positive Purkinje cells are organized in the cerebellum as stripes or bands that run from anterior to posterior across the cerebellum and alternate with bands of Aldolase C-negative Purkinje cells (2). Aldolase C is also expressed in astrocytes (3)