Neuron-glial antigen2 antibody - 481 005

The NG2 is a valuable marker for several incompletely differentiated precursor cells
Guinea pig polyclonal purified antibody
Cat. No.: 481 005
Amount: 50 µg
Price: $460.00
Cat. No. 481 005 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: yes
ICC: 1 : 500 (see remarks) gallery  
IHC: not recommended
IHC-P: not recommended
IHC-Fr: 1 : 500 (see remarks) gallery  
IHC-G: 1 : 500 (see remarks) gallery  
Immunogen Recombinant protein corresponding a part of the extracellular domain of mouse neuron-glial antigen 2. (UniProt Id: Q8VHY0)
Reactivity Reacts with: mouse (Q8VHY0), rat (Q00657).
Other species not tested yet.
Remarks

ICC: Methanol fixation is recommended.
IHC-Fr: Methanol fixation is recommended.
IHC-G: Fixation with 9% glyoxal, 8% acetic acid in ddH2O according to Konno et al. 2023 is recommended.

Data sheet 481_005.pdf
Cat. No.: 481 005
Amount: 50 µg
Price: $460.00
Background

The NG2 proteoglycan is a type I membrane protein that is expressed by a variety of immature cells of several embryonic tissue origins including glia, muscle progenitor cells, and pericytes (1). In the central nervous system, expression of NG2 was originally thought to specify oligodendroglial progenitor cells, but more recent data suggest that NG2-expressing cells encompass a wider range of immature glial cells in white and gray matter. These include glia that make synaptic-like contacts with neurons in the hippocampus and cerebellum (2) and glial cells specifically associated with the nodes of Ranvier (3). Interestingly, many NG2-positive cells are both proliferative and motile or exhibit local process motility (4, 5).