| Cat. No. 482 005 |
50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Immunogen | Recombinant protein corresponding to residues near the carboxy terminus of mouse Claudin5. (UniProt Id: O54942) |
| Reactivity |
Reacts with: rat (Q9JKD6), mouse (O54942), dog. Other species not tested yet. |
| Remarks |
ICC: Methanol fixation is recommended |
| Data sheet | 482_005.pdf |
Claudin5 is predominantly expressed in endothelial cells, especially brain endothelial cells (1) and therefore it is thought that the paracellular permeability of the blood–brain barrier is largely determined by the expression levels of Claudin5. An initial study using Claudin5 knockout (KO) mice clearly showed that expression of Claudin5 in the blood–brain barrier is essential for preventing the entrance of molecules with molecular weights between 400 and 800 Da in the brain. (2) The size-selective modulation of blood–brain barrier permeability has an advantage in comparison to clinically performed methods for increasing blood–brain barrier permeability, namely intra-carotid hyperosmolar mannitol administration. Mannitol administration completely disrupts the blood–brain barrier by withdrawing water from endothelial cells and enabling the entrance of bloodborne proteins into the brain. (3,4)