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Vimentin antibody - HS-172 011

Vimentin is a type III intermediate filament
Mouse monoclonal purified IgG
Cat. No.: HS-172 011
Amount: 100 µg
Price: $415.00
Cat. No. HS-172 011 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP staining) gallery  
IP: not tested yet
ICC: 1 : 500 gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
Clone V9
Subtype IgG1 (κ light chain)
Immunogen Full-length pig Vimentin recombinant protein (UniProt Id: P02543)
Reactivity Reacts with: human (P08670), rat (P31000).
No signal: mouse (P20152).
Other species not tested yet.
Data sheet hs-172_011.pdf
Cat. No.: HS-172 011
Amount: 100 µg
Price: $415.00

Vimentin (red) and Synaptopodin (green) staining in glomeruli of rat kidney

 

WB
ICC
IHC
IHC
IHC-P
IHC-P
IHC-P
IHC-P
Background
Vimentin belongs to the family of intermediate filaments that can be subdivided into six major groups based on sequence similarity. Vimentin belongs to the type III category and is the predominant subunit protein of intermediate filaments in tissues of mesenchymal origin.
Like other intermediate filaments it plays a role in the cytoskeletal organization and maintenance of cell shape and morphology.
Protocols for HS-172 011
WB
ICC
IHC
IHC-P
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