IBA1 antibody - HS-234 013 K.O.

IBA1 is a marker for macrophages and microglia
Rabbit polyclonal purified antibody
Cat. No.: HS-234 013
Amount: 200 µl
Price: $380.00
Cat. No. HS-234 013 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IHC: 1 : 200 gallery  
IHC-P: 1 : 100 gallery  
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity K.O. validated
Data sheet hs-234_013.pdf

References for IBA1 - HS-234 013

Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy.
Gáll Z, Kelemen K, Tolokán A, Zolcseak I, Sável I, Bod R, Ferencz E, Vancea S, Urkon M, Kolcsár M
Biomedicines (2022) 108: . HS-234 013 IHC; tested species: mouse
Cat. No.: HS-234 013
Amount: 200 µl
Price: $380.00

IBA 1 positive cells in FFPE mouse small intestine section

Anticonvulsant Action and Long-Term Effects of Chronic Cannabidiol Treatment in the Rat Pentylenetetrazole-Kindling Model of Epilepsy.
Gáll Z, Kelemen K, Tolokán A, Zolcseak I, Sável I, Bod R, Ferencz E, Vancea S, Urkon M, Kolcsár M
Biomedicines (2022) 108: . HS-234 013 IHC; tested species: mouse
Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3). 

Protocols for HS-234 013