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IBA1 antibody - HS-234 017

IBA1 is a marker for macrophages and microglia
Monoclonal rat purified IgG
Cat. No.: HS-234 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-234 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: 1 : 100 up to 1 : 200 gallery  
IHC: 1 : 100 up to 1 : 200 gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 200 gallery  
Clone 134G9
Subtype IgG2b (λ light chain)
Immunogen Synthetic peptide corresponding to AA 131 to 146 from mouse IBA1 (UniProt Id: Q9EQW9)
Reactivity Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008).
Other species not tested yet.
Specificity Specific for IBA 1
Data sheet hs-234_017.pdf
Cat. No.: HS-234 017
Quantity: 200 µl
Price: $415.00

Staining of murine spleen section with rat anti-IBA1 and rabbit anti-F4/80

Background

Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).