| Cat. No. HS-234 017BT |
100 µg purified IgG, lyophilized, labeled with
Biotin.
Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Label | biotin |
| Clone | 134G9 |
| Subtype | IgG2b (λ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse IBA1 (UniProt Id: Q9EQW9) |
| Reactivity |
Reacts with: mouse (Q9EQW9), rat (P55009), human (P55008). Other species not tested yet. |
| Data sheet | hs-234_017bt.pdf |
Staining of murine spleen section with biotinylated rat anti-IBA1
Ionized calcium-binding adaptor molecule 1 (IBA1) or allograft inflammatory factor1 (AIF-1) is an EF hand calcium binding protein which is expressed by cells of the monocyte/macrophage lineage and by germ cells in the testis (1). In mice, IBA1/AIF-1 can be regarded a “pan-macrophage marker” because, except for alveolar macrophages, all subpopulations of macrophages express IBA1/AIF-1 (1). In human gliomas IBA1 defines a distinct subset of tumor-associated activated macrophages/microglial cells (2).
Microglia represent the resident macrophages in the nervous system and are the smallest of the glial cells with cell bodies of only 2-5 µm in diameter. In the CNS IBA1 upregulation is associated with neuroinflammatory response (3).