CD31 antibody mouse specific - HS-351 008

CD31 is an endothelial cell marker
Rabbit monoclonal recombinant IgG
Cat. No.: HS-351 008
Amount: 50 µg
Price: $415.00
Cat. No. HS-351 008 50 µg purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 2000 up to 1 : 5000 (see remarks) gallery  
IHC-P: 1 : 1000 up to 1 : 4000 gallery  
Clone Rb53A2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues surrounding AA 680 of mouse CD31 (UniProt Id: Q08481)
Reactivity Reacts with: mouse (Q08481), rat (Q3SWT0).
No signal: human (P16284).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody SY-53A2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. The antibody can therefore be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Heat-mediated antigen retrieval (citrate buffer pH 6) can improve immunohistochemical staining

Data sheet hs-351_008.pdf
Cat. No.: HS-351 008
Amount: 50 µg
Price: $415.00

Mouse liver section stained with CD31 (red) and F4/80 (green)

Background

CD31 or platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a cell adhesion molecule that contains a single trans-membrane domain and 6 Ig-like C2-type (immunoglobulin-like) domains (1). It is expressed on the surface of leukocytes and platelets and is primarily concentrated at endothelial cell-cell junctions (2). CD31 plays a role in several important biological processes, such as vascular development, maintenance of vascular endothelial barrier function and leukocyte emigration at inflammatory sites. In addition, CD31 promotes cancer metastasis by inducing epithelial-mesenchymal transition (3).