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CD 4 - HS-360 117

A T-cell surface glycoprotein
Monoclonal rat purified IgG
Cat. No.: HS-360 117
Amount: 200 µl
Price: 415.00 €
Cat. No. HS-360 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 gallery  
IHC: 1 : 100 (see remarks) gallery  
IHC-P/FFPE: 1 : 100 gallery  
Clone 78H9D2
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730).
Other species not tested yet.
Specificity Specific for CD 4
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet hs-360_117.pdf
Cat. No.: HS-360 117
Quantity: 200 µl
Price: 415.00 €

Indirect immunostaining of formalin-fixed paraffin-embedded mouse spleen section with monoclonal rat anti-CD4 (cat. no.: HS-360 117; red) and rabbit polyclonal anti-CD3e (cat. no.: HS-413 103; green) demonstrating the co-localization in CD4 + T-cells (overlay: yellow). Nuclei have been visualized by DAPI staining (blue).

Background
CD 4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD 4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell.