|Cat. No. HS-360 117||200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 gallery
IHC: 1 : 100 (see remarks) gallery
IHC-P/FFPE: 1 : 100 gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)|
Reacts with: mouse (P06332).
No signal: human (P01730).
Other species not tested yet.
|Specificity||Specific for CD4|
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.
Indirect immunostaining of formalin-fixed paraffin-embedded mouse spleen section with monoclonal rat anti-CD4 (cat. no.: HS-360 117; red) and rabbit polyclonal anti-CD3e (cat. no.: HS-413 103; green) demonstrating the co-localization in CD4 + T-cells (overlay: yellow). Nuclei have been visualized by DAPI staining (blue).
CD 4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD 4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell.