|Cat. No. HS-360 117||
200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 100 (see remarks) gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 100 gallery
Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-Fr: 1 : 100 (see remarks) gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)|
Reacts with: mouse (P06332).
No signal: human (P01730), rat (P05540).
Other species not tested yet.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.
Indirect immunostaining of formalin-fixed paraffin-embedded mouse spleen section with monoclonal rat anti-CD4 (cat. no.: HS-360 117; red) and rabbit polyclonal anti-CD3e (cat. no.: HS-413 103; green) demonstrating the co-localization in CD4 + T-cells (overlay: yellow). Nuclei have been visualized by DAPI staining (blue).
CD 4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD 4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell.