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CD4 antibody mouse specific - HS-360 117

CD4 is a T-cell surface glycoprotein
Rat monoclonal purified IgG
Cat. No.: HS-360 117
Amount: 200 µl
Price: $420.00
Cat. No. HS-360 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 (see remarks) gallery  
IHC-P: 1 : 100 gallery  
IHC-Fr: 1 : 100 (see remarks) gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.

Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 78H9D2
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730), rat (P05540).
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
IHC-Fr: PFA and MeOH fixation are possible.
Data sheet hs-360_117.pdf

References for CD4 - HS-360 117

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. HS-360 117 IHC; tested species: mouse
An Enhancer Variant Associated with Breast Cancer Susceptibility in Black Women Regulates TNFSF10 Expression and Antitumor Immunity in Triple-Negative Breast Cancer.
Han YJ, Zhang J, Hardeman A, Liu M, Karginova O, Romero R, Khramtsova GF, Zheng Y, Huo D, Olopade OI
Human molecular genetics (2022) : . HS-360 117 IHC-P; tested species: mouse
Cat. No.: HS-360 117
Amount: 200 µl
Price: $420.00

Indirect immunostaining of formalin-fixed paraffin-embedded mouse spleen section with monoclonal rat anti-CD4 (cat. no.: HS-360 117; red) and rabbit polyclonal anti-CD3e (cat. no.: HS-413 103; green) demonstrating the co-localization in CD4 + T-cells (overlay: yellow). Nuclei have been visualized by DAPI staining (blue).

INSIHGT: an accessible multi-scale, multi-modal 3D spatial biology platform.
Yau CN, Hung JTS, Campbell RAA, Wong TCY, Huang B, Wong BTY, Chow NKN, Zhang L, Tsoi EPL, Tan Y, Li JJX, et al.
Nature communications (2024) 151: 10888. HS-360 117 IHC; tested species: mouse
An Enhancer Variant Associated with Breast Cancer Susceptibility in Black Women Regulates TNFSF10 Expression and Antitumor Immunity in Triple-Negative Breast Cancer.
Han YJ, Zhang J, Hardeman A, Liu M, Karginova O, Romero R, Khramtsova GF, Zheng Y, Huo D, Olopade OI
Human molecular genetics (2022) : . HS-360 117 IHC-P; tested species: mouse
WB
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-Fr
Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD4 is a co-receptor that assists the T-cell receptor (TCR) in communicating with an antigen-presenting cell.
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