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CD4 antibody mouse specific - HS-360 117

CD4 is a T-cell surface glycoprotein
Rat monoclonal purified IgG
Cat. No.: HS-360 117
Amount: 200 µl
Price: $415.00
Cat. No. HS-360 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 100 (see remarks) gallery  
IHC-P: 1 : 100 gallery  
IHC-Fr: 1 : 100 (see remarks) gallery  
Clone 78H9D2
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730), rat (P05540).
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
IHC-Fr: PFA and MeOH fixation are possible.
Data sheet hs-360_117.pdf

References for CD4 - HS-360 117

An Enhancer Variant Associated with Breast Cancer Susceptibility in Black Women Regulates TNFSF10 Expression and Antitumor Immunity in Triple-Negative Breast Cancer.
Han YJ, Zhang J, Hardeman A, Liu M, Karginova O, Romero R, Khramtsova GF, Zheng Y, Huo D, Olopade OI
Human molecular genetics (2022) : . HS-360 117 IHC-P; tested species: mouse
Cat. No.: HS-360 117
Amount: 200 µl
Price: $415.00

Indirect immunostaining of formalin-fixed paraffin-embedded mouse spleen section with monoclonal rat anti-CD4 (cat. no.: HS-360 117; red) and rabbit polyclonal anti-CD3e (cat. no.: HS-413 103; green) demonstrating the co-localization in CD4 + T-cells (overlay: yellow). Nuclei have been visualized by DAPI staining (blue).

An Enhancer Variant Associated with Breast Cancer Susceptibility in Black Women Regulates TNFSF10 Expression and Antitumor Immunity in Triple-Negative Breast Cancer.
Han YJ, Zhang J, Hardeman A, Liu M, Karginova O, Romero R, Khramtsova GF, Zheng Y, Huo D, Olopade OI
Human molecular genetics (2022) : . HS-360 117 IHC-P; tested species: mouse
WB
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-Fr
Background

CD 4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD 4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell.
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