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CD4 antibody - HS-360 117BT

CD4 is a T-cell surface glycoprotein
Rat monoclonal purified antibody
Cat. No.: HS-360 117BT
Amount: 100 µg
Price: $465.00
Cat. No. HS-360 117BT 100 µg specific antibody, lyophilized. Affinity purified with the immunogen, labeled with Biotin.

Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality.
Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems.
The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

 Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 500 (AP-Staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 50 gallery  
Label biotin
Clone 78H9D2
Subtype IgG2b (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730), rat (P05540).
Other species not tested yet.
Data sheet hs-360_117bt.pdf
Cat. No.: HS-360 117BT
Amount: 100 µg
Price: $465.00

Staining of murine spleen section with biotinylated rat anti-CD4

WB
IHC-P
Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD4 is a co-receptor that assists the T-cell receptor (TCR) in communicating with an antigen-presenting cell.
Protocols for HS-360 117BT
WB
IHC-P
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