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CD4 antibody - HS-360 308

CD4 is a T-cell surface glycoprotein
Guinea pig monoclonal recombinant IgG
Cat. No.: HS-360 308
Amount: 100 µl
Price: $415.00
Cat. No. HS-360 308 100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 1000 gallery  
Clone Gp78H9D2
Subtype IgG2 (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730).
Other species not tested yet.
Specificity Specific for CD4
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 78H9D2. The constant regions of the heavy and light chains have been replaced by Guinea-pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet hs-360_308.pdf
Cat. No.: HS-360 308
Amount: 100 µl
Price: $415.00

CD4 positive T-cells in FFPE mouse colon section

Background

CD 4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD 4 is a co-receptor that assists the T cell receptor (TCR) in communicating with an antigen-presenting cell.

Protocols