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CD4 antibody mouse specific - HS-360 308

CD4 is a T-cell surface glycoprotein
Guinea pig monoclonal recombinant IgG
Cat. No.: HS-360 308
Amount: 100 µl
Price: $415.00
Cat. No. HS-360 308 100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 up to 1 : 2000 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone Gp78H9D2
Subtype IgG2 (κ light chain)
Immunogen Recombinant protein corresponding to AA 27 to 394 from mouse CD4 (UniProt Id: P06332)
Reactivity Reacts with: mouse (P06332).
No signal: human (P01730).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 78H9D2. The constant regions of the heavy and light chains have been replaced by Guinea-pig specific sequences. Therefore, the antibody can be used with standard anti-Guinea pig secondary reagents. The antibody has been expressed in mammalian cells.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.
Data sheet hs-360_308.pdf
Cat. No.: HS-360 308
Amount: 100 µl
Price: $415.00

CD4 positive T-cells in FFPE mouse colon section

WB
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
Background

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages, and dendritic cells.
CD4 is a co-receptor that assists the T-cell receptor (TCR) in communicating with an antigen-presenting cell.
Protocols for HS-360 308
WB
IHC
IHC-P
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