CD8a antibody mouse specific - HS-361 008

CD8a is a T-cell surface glycoprotein

Rabbit monoclonal recombinant IgG

Cat. No.: HS-361 008

Amount: 100 µl

Price: $415.00

Cat. No. HS-361 008	100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 1000 (AP-staining) gallery IP: not tested yet ICC: not tested yet IHC: not tested yet IHC-P: 1 : 100 gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	Rb321E9
Subtype	IgG1 (κ light chain)
Immunogen	Synthetic peptide corresponding to AA 230 to 247 from mouse CD8a (UniProt Id: P01731)
Reactivity	Reacts with: mouse (P01731). No signal: human, rat. Other species not tested yet.
Remarks	This antibody is a chimeric antibody based on the monoclonal rat antibody clone GHH8/32. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
Data sheet	hs-361_008.pdf

References for CD8a - HS-361 008

Protein Kinase Inhibitor-Mediated Immunoprophylactic and Immunotherapeutic Control of Colon Cancer.
Ghione S, Racoeur C, Mabrouk N, Shan J, Groetz E, Ballot E, Truntzer C, Chouchane L, Végran F, Paul C, Plenchette S, et al.
Frontiers in immunology (2022) 13: 875764. HS-361 008 IHC-P; tested species: mouse

Cat. No.: HS-361 008

Amount: 100 µl

Price: $415.00

CD8a and CD4 positive T-cell populations in FFPE mouse spleen section

IHC-P

Background

CD 8 is a heterodimeric T-cell surface glycoprotein that consists of a CD 8a (alpha) and beta chain. It identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD 8 is thought to play a role in the process of T-cell mediated killing. CD 8 alpha chains binds to class I MHC molecules alpha-3 domains.

1 ) Frost EL, Journal of immunology (Baltimore, Md. : 1950) (2015) 1958: 3520-4.
2 ) Wu RC, Oncoimmunology (2012) 16: 954-956.
3 ) Hyland L, Virology (1994) 2041: 234-41.
4 ) Prince HE, Diagnostic and clinical immunology (1987) 54: 188-93.
5 ) Gaudernack G, Journal of immunological methods (1986) 902: 179-87.

Protocols for HS-361 008

AP Detection

IHC-P

Chromogen detection

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