CD8a antibody mouse specific - HS-361 017

CD8a is a T-cell surface glycoprotein

Rat monoclonal purified IgG

Cat. No.: HS-361 017

Amount: 200 µl

Price: $415.00

Cat. No. HS-361 017	200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H₂O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications	WB: 1 : 500 (AP-staining) gallery IP: not tested yet IHC: not tested yet IHC-P: 1 : 100 gallery IHC-Fr: 1 : 200 (see remarks) gallery Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section. Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section. Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section. Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Immunohistochemistry on fresh frozen (IHC-Fr) cryo-tissue-sections. In contrast to standard PFA perfusion fixed tissues, fresh frozen cryo-tissue-sections can be variably postfixed with alcohols, acetone or PFA. Alcohol or acetone fixation is e.g. of advantage for antigens masked by PFA crosslinking. For recommended postfixation, please refer to the ”Remarks” section. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone	GHH8/321E9
Subtype	IgG2a
Immunogen	Synthetic peptide corresponding to AA 230 to 247 from mouse CD8a (UniProt Id: P01731)
Reactivity	Reacts with: mouse (P01731). No signal: human, rat. Other species not tested yet.
Remarks	IHC-Fr: PFA fixation is recommended.
Data sheet	hs-361_017.pdf

References for CD8a - HS-361 017

Impact of Chemical-Induced Mutational Load Increase on Immune Checkpoint Therapy in Poorly Responsive Murine Tumors.
Kuczynski EA, Krueger J, Chow A, Xu P, Man S, Sundaravadanam Y, Miller JK, Krzyzanowski PM, Kerbel RS
Molecular cancer therapeutics (2018) 174: 869-882. HS-361 017 IHC-P; tested species: mouse

Helicobacter pylori γ-glutamyl transferase contributes to colonization and differential recruitment of T cells during persistence.
Wüstner S, Anderl F, Wanisch A, Sachs C, Steiger K, Nerlich A, Vieth M, Mejías-Luque R, Gerhard M
Scientific reports (2017) 71: 13636. HS-361 017 IHC-P; tested species: mouse

Cat. No.: HS-361 017

Amount: 200 µl

Price: $415.00

CD8 positive T-cells in FFPE mouse thymus section

IHC-P

IHC-Fr

Background

CD 8 is a heterodimeric T-cell surface glycoprotein that consists of a CD 8a (alpha) and beta chain. It identifies cytotoxic/suppressor T-cells that interact with MHC class I bearing targets. CD 8 is thought to play a role in the process of T-cell mediated killing. CD 8 alpha chains binds to class I MHC molecules alpha-3 domains.

1 ) Frost EL, Journal of immunology (Baltimore, Md. : 1950) (2015) 1958: 3520-4.
2 ) Wu RC, Oncoimmunology (2012) 16: 954-956.
3 ) Hyland L, Virology (1994) 2041: 234-41.
4 ) Prince HE, Diagnostic and clinical immunology (1987) 54: 188-93.
5 ) Gaudernack G, Journal of immunological methods (1986) 902: 179-87.

Protocols for HS-361 017

AP Detection

IHC-P

Chromogen detection

IHC-Fr

Immunostaining with Formaldehyde

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