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STAT6 antibody - HS-378 011

STAT6 is a marker for solitary fibrous tumors
Mouse monoclonal purified IgG
Cat. No.: HS-378 011
Amount: 100 µg
Price: $415.00
Cat. No. HS-378 011 100 µg purified IgG, lyophilized. Azide was added before lyophilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not tested yet
IP: not tested yet
ICC: not tested yet
IHC: not tested yet
IHC-P: 1 : 200 up to 1 : 1000 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Clone 37C12
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 832 to 847 from human Stat6 (UniProt Id: P42226)
Reactivity Reacts with: human (P42226).
Other species not tested yet.
Data sheet hs-378_011.pdf
Cat. No.: HS-378 011
Amount: 100 µg
Price: $415.00

Nuclear STAT6 expression in hemangiopericytoma

IHC-P
Background

STAT 6 (Signal Transducer and Activator of Transcription 6) is a member of the STAT family of transcription factors. Gene fusion of NGFI-A binding protein 2 (NAB 2) with STAT 6, which converts the transcriptional repressor NAB 2 into a transcriptional activator, was detected as driver mutation in solitary fibrous tumors (SFT). The NAB 2-STAT 6 fusion shows a consistent nuclear reallocation in STAT 6 immunohistochemistry. STAT 6 is a highly sensitive and specific immunohistochemical marker for most SFT and can be helpful to distinguish this tumor type from histologic mimics.
Although considered to be a distinct entity from meningeal SFT in the WHO Classification of CNS Tumours (2007), nuclear STAT 6 expression is additionally found in meningeal hemangiopericytoma (HPC). STAT 6 is a reliable marker to distinguish meningeal solitary fibrous tumors-hemangiopericytomas versus meningiomas.
This antibody is part of the HistoSure® product line, specifically developed and tested for human pathology.