|Cat. No. HS-384 017||200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 100 up to 1 : 100 (see remarks) gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 1141 to 1152 from human CD11b (UniProt Id: P11215)|
Epitop: AA 1141 to 1152 from human CD11b (UniProt Id: P11215)
Reacts with: human (P11215).
No signal: mouse (P05555).
Other species not tested yet.
|Specificity||Specific for human CD11b|
IHC-P: This antibody shows weak non-specific cross-reactivity to murine macrophages after antigen retrieval at very high temperatures (121°, pressure cooker). Use appropriate controls when optimizing your IHC-P protocol for detection of human cells in humanized mouse models.
Indirect immunostaining of human lung section with rat anti-CD11b detects low CD11b expression in alveolar macrophages.
CD 11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells. Antibodies against CD 11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).