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CD11b antibody - HS-384 017

CD11b is a component of the macrophage-1 antigen (Mac-1)
Monoclonal rat purified IgG
Cat. No.: HS-384 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-384 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications WB: (AP-staining) gallery  
IHC-P/FFPE: 1 : 100 up to 1 : 100 (see remarks) gallery  
Clone 194B8D5
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 1141 to 1152 from human CD11b (UniProt Id: P11215)
Epitop Epitop: AA 1141 to 1152 from human CD11b (UniProt Id: P11215)
Reactivity Reacts with: human (P11215).
No signal: mouse (P05555).
Other species not tested yet.
Specificity Specific for human CD11b
Remarks

IHC-P: This antibody shows weak non-specific cross-reactivity to murine macrophages after antigen retrieval at very high temperatures (121°, pressure cooker). Use appropriate controls when optimizing your IHC-P protocol for detection of human cells in humanized mouse models.

Data sheet hs-384_017.pdf
Cat. No.: HS-384 017
Quantity: 200 µl
Price: $415.00

Indirect immunostaining of human lung section with rat anti-CD11b detects low CD11b expression in alveolar macrophages.

Background

CD 11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells.  Antibodies against CD 11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

Protocols