CD11b antibody human specific - HS-384 018

CD11b is a component of the macrophage-1 antigen (Mac-1)
Rabbit monoclonal recombinant IgG
Cat. No.: HS-384 018
Amount: 100 µl
Price: $415.00
Cat. No. HS-384 018 100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IHC-P: 1 : 400 up to 1 : 1000 gallery  
Clone Rb194B8D5
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of human CD11b (UniProt Id: P11215)
Reactivity Reacts with: human (P11215).
No signal: mouse (P05555).
Other species not tested yet.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 194B8D5. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.

Data sheet hs-384_018.pdf
Cat. No.: HS-384 018
Amount: 100 µl
Price: $415.00

Rabbit anti-human CD11b staines only CD11b-positive cells in human tonsil. It does not stain CD11b in murine CD11b-positive cells in mouse spleen.

Background

CD11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells. Antibodies against CD11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

Protocols for HS-384 018