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CD11b - HS-384 103

A component of the macrophage-1 antigen (Mac-1)
Polyclonal rabbit purified antibody
Cat. No.: HS-384 103
Amount: 200 µl
Price: $450.00
Cat. No. HS-384 103 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 1000 gallery  
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 250 gallery  
Immunogen Synthetic peptide corresponding to AA 999 to 1016 from mouse CD11b (UniProt Id: P05555)
Reactivity Reacts with: mouse (P05555).
No signal: human (P11215).
Other species not tested yet.
Specificity Specific for CD 11b
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings. Antibody incubation over-night at room temperature is recommended.

Data sheet hs-384_103.pdf
Cat. No.: HS-384 103
Quantity: 200 µl
Price: $450.00

Increased CD11b expression represents microglial activation in a mouse model of Alzheimer's Disease.

Background

CD 11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells.  Antibodies against CD 11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

Protocols