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CD11b antibody - HS-384 117

CD11b is a component of the macrophage-1 antigen (Mac-1)
Rat monoclonal purified IgG
Cat. No.: HS-384 117
Amount: 200 µl
Price: $415.00
Cat. No. HS-384 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications
 
WB: 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 200 gallery  
Clone 298G2H5
Subtype IgG2a (κ light chain)
Epitop Epitop: AA 999 to 1016 from mouse CD11b (UniProt Id: P05555)
Reactivity Reacts with: mouse (P05555).
No signal: human (P11215).
Other species not tested yet.
Specificity Specific for CD 11b
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings. Antibody incubation over-night at room temperature is recommended.

Data sheet hs-384_117.pdf

References for CD11b - HS-384 117

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-384 117 IHC-P; tested species: mouse
Cat. No.: HS-384 117
Amount: 200 µl
Price: $415.00

Increased CD11b expression represents microglial activation in virus infected mouse brain.

Influenza A Virus (H1N1) Infection Induces Microglial Activation and Temporal Dysbalance in Glutamatergic Synaptic Transmission.
Düsedau HP, Steffen J, Figueiredo CA, Boehme JD, Schultz K, Erck C, Korte M, Faber-Zuschratter H, Smalla KH, Dieterich D, Kröger A, et al.
mBio (2021) 125: e0177621. HS-384 117 IHC-P; tested species: mouse
Background

CD 11b also called integrin alpha-M (ITGAM) is one protein subunit that forms together with CD18 the heterodimeric integrin αMβ2 complex, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3) (1). αMβ2 is expressed on polymorphonuclear neutrophils (PMN), monocytes, macrophages, some subsets of cytotoxic T lymphocytes, and NK cells.  Antibodies against CD 11b are frequently used to identify macrophages and microglia, however not all tissue-resident macrophages are CD11b positive. In the murine liver CD11b expression is rare and almost exclusively found on F4/80 negative cells (2). In the murine spleen CD11b+ cells are less numerous than F4/80+ cells, co-expression of CD11b and F4/80 is also rare and CD11b+ cells tend to be closer to the marginal zone (2). CD11b upregulation on residential alveolar macrophages is a marker of acute and chronic lung inflammation in mice (3). Also, in the brain CD11b is markedly increased during microglial activation (4).

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