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F4/80 antibody - HS-397 004

F4/80 is a marker protein for murine macrophages
Guinea pig polyclonal antiserum
Cat. No.: HS-397 004
Amount: 100 µl
Price: $350.00
Cat. No. HS-397 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: not recommended
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 500 gallery  

Western blot (WB); separation of proteins by PAGE and subsequent transfer to a membrane. Detection of target molecules is carried out with antibodies. Some antibodies require special sample preparation steps. For details, please refer to the “Remarks” section.

Immunoprecipitation (IP); Immunoisolation or pulldown of a target molecule using an antibody. For details and product specific hints, please refer to the ”Remarks” section.

Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.

Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.

Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
Other species not tested yet.
Data sheet hs-397_004.pdf

References for F4/80 - HS-397 004

Zika virus infection causes widespread damage to the inner ear.
Yee KT, Neupane B, Bai F, Vetter DE
Hearing research (2020) 395: 108000. HS-397 004 IHC; tested species: mouse
Cat. No.: HS-397 004
Amount: 100 µl
Price: $350.00

F4/80 positive cells in FFPE mouse liver section

Zika virus infection causes widespread damage to the inner ear.
Yee KT, Neupane B, Bai F, Vetter DE
Hearing research (2020) 395: 108000. HS-397 004 IHC; tested species: mouse
IHC
IHC-P
IHC-P
IHC-P
Background
The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.
Protocols for HS-397 004
IHC
IHC-P
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