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F4/80 - HS-397 004

A marker protein for murine macrophages
Polyclonal Guinea pig antiserum
Cat. No.: HS-397 004
Amount: 100 µl
Price: 350.00 €
Cat. No. HS-397 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Applications WB: not recommended
IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 500 gallery  
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
Other species not tested yet.
Specificity Specific for F4/80.
Data sheet hs-397_004.pdf

References for F4/80 - HS-397 004

Zika virus infection causes widespread damage to the inner ear.
Yee KT, Neupane B, Bai F, Vetter DE
Hearing research (2020) 395: 108000. HS-397 004 IHC; tested species: mouse
Cat. No.: HS-397 004
Quantity: 100 µl
Price: 350.00 €

F4/80 positive cells in FFPE mouse liver section

Zika virus infection causes widespread damage to the inner ear.
Yee KT, Neupane B, Bai F, Vetter DE
Hearing research (2020) 395: 108000. HS-397 004 IHC; tested species: mouse
Background
The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.
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