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F4/80 antibody - HS-397 008

F4/80 is a marker protein for murine macrophages
Monoclonal Recombinant rabbit purified IgG
Cat. No.: HS-397 008
Amount: 100 µl
Price: $415.00
Cat. No. HS-397 008 100 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications IHC: 1 : 500 gallery  
IHC-P/FFPE: 1 : 100 (see remarks) gallery  
Clone Rb167B3
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Epitop Epitop: AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
Other species not tested yet.
Specificity Specific for F4/80
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone 167B3. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-P: In contrast to the original rat antibody clone 167B3, the rabbit chimeric antibody (cat. no. HS-397 008) shows weak non-specific staining of nucleoli in mouse brain using the SYSY Reference protocol.

Data sheet hs-397_008.pdf
Cat. No.: HS-397 008
Quantity: 100 µl
Price: $415.00

F4/80 positive Kupffer cells in FFPE mouse liver. Immunohistochemical staining of a formalin fixed paraffin embedded mouse liver tissue section with monoclonal rabbit anti-F4/80 (cat, no. HS-397 008, dilution 1:100, DAB). Nuclei have been counterstained with haematoxylin (blue).

Background
The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.
Protocols