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F4/80 antibody - HS-397 017

F4/80 is a marker protein for murine macrophages
Rat monoclonal purified IgG
Cat. No.: HS-397 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-397 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 250 (AP staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 gallery  
IHC-P: 1 : 100 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone 167B3
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to AA 28 to 42 from mouse F4/80 (UniProt Id: Q61549)
Reactivity Reacts with: mouse (Q61549).
No signal: rat.
Other species not tested yet.
Remarks

IHC-Fr: PFA and MeOH fixation are recommended.
Data sheet hs-397_017.pdf
Cat. No.: HS-397 017
Amount: 200 µl
Price: $415.00

F4/80 positive cells in murine FFPE tissues

WB
IHC
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-P
IHC-Fr
Background
The murine macrophage cell surface glycoprotein F4/80 is a member of the epidermal growth factor-seven transmembrane (EGF-TM7) family. It is involved in the generation of antigen-specific efferent regulatory T cells that suppress antigen-specific immunity but not required for the development and distribution of tissue macrophages.
Although F4/80 is widely used as a marker of murine macrophage populations it is not equivalently expressed across tissue-specific macrophage lineages: e.g. red pulp macrophages of the spleen and Kupffer cells of the liver are F4/80-positive, white pulp and marginal zone macrophages of the spleen are F4/80-negative and alveolar macrophages are F4/80dim.
F4/80 expression is not restricted to macrophages, but also found in murine Epidermal Langerhans Cells; dendritic cells of the skin. The human ortholog of F4/80, EGF-like module containing mucin-like hormone receptor (EMR)1, is absent on mononuclear phagocytic cells including monocytes, macrophages, and myeloid dendritic cells and seems to be highly specific for eosinophils in humans.
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