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Lamin B1 - HS-404 003

A nuclear lamina protein
Polyclonal rabbit purified antibody
Cat. No.: HS-404 003
Amount: 200 µl
Price: 370.00 €
Cat. No. HS-404 003 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C until use.
Applications WB: 1 : 1000 gallery  
ICC: 1 : 100 gallery  
IHCgallery  
IHC-P/FFPE: 1 : 100 gallery  
Immunogen Synthetic peptide corresponding to AA 6 to 19 from mouse LaminB1 (UniProt Id: P14733)
Reactivity Reacts with: mouse (P14733).
No signal: human (P20700).
Other species not tested yet.
Specificity Specific for Lamin B1.
Data sheet hs-404_003.pdf
Cat. No.: HS-404 003
Quantity: 200 µl
Price: 370.00 €

Detection of nuclear Lamin B1 in cells of human (brown) and murine (red) origin in various xenograft models 

Background

Lamin B1 (LMNB1) is an intermediate filament-type protein of the nuclear lamina and is ubiquitously expressed throughout development. It plays important roles in many cellular processes like the distribution of heterochromatin and the regulation of gene expression and splicing. The maintenance of LMNB1 protein levels is required for DNA replication and repair and thus mutations in B-type lamins are usually lethal.
Duplication of the LMNB1 gene causes adult-onset autosomal-dominant leukodystrophy (ADLD), a rare neurological disorder in which overexpression of LMNB1 causes progressive central nervous system demyelination. Improper Lamin B1 expression is often present in tumor cells and decreased levels are observed for example in colon cancer, breast cancer and B-cell malignancies. Lamin B1 loss is also a senescence-associated biomarker and distinguishes senescent from proliferating cells in pre-neoplastic lesions or marks senescent cells in various age-related pathologies.

These antibody are suited to distinguish murine from human cells in humanized mouse models.