| Cat. No. HS-404 018 |
100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | Rb12G6 |
| Subtype | IgG1 (κ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the amino terminus of human Lamin B1 (UniProt Id: P20700) |
| Reactivity |
Reacts with: human (P20700), pig. No signal: mouse (P14733), rat (P70615). Other species not tested yet. |
| Remarks |
This antibody is a chimeric antibody based on the monoclonal rat antibody clone 12G6. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells. |
| Data sheet | hs-404_018.pdf |
Red staining of Lamin B1 in human tumor cells in a xenograft model
Lamin B1 (LMNB1) is an intermediate filament-type protein of the nuclear lamina and is ubiquitously expressed throughout development. It plays important roles in many cellular processes like the distribution of heterochromatin and the regulation of gene expression and splicing. The maintenance of LMNB1 protein levels is required for DNA replication and repair and thus mutations in B-type lamins are usually lethal.
Duplication of the LMNB1 gene causes adult-onset autosomal-dominant leukodystrophy (ADLD), a rare neurological disorder in which overexpression of LMNB1 causes progressive central nervous system demyelination. Improper Lamin B1 expression is often present in tumor cells and decreased levels are observed for example in colon cancer, breast cancer and B-cell malignancies. Lamin B1 loss is also a senescence-associated biomarker and distinguishes senescent from proliferating cells in pre-neoplastic lesions or marks senescent cells in various age-related pathologies.
These antibody are suited to distinguish murine from human cells in humanized mouse models.