| Cat. No. HS-404 117 |
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Clone | SY-4H6 |
| Subtype | IgG2b (κ light chain) |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of mouse Lamin B1 (UniProt Id: P14733) |
| Reactivity |
Reacts with: mouse (P14733), pig. No signal: human (P20700), rat (P70615). Other species not tested yet. |
| Remarks |
ICC: Methanol fixation is recommended. |
| Data sheet | hs-404_117.pdf |
Chromogenic double staining of nuclear lamina proteins identifies mouse (brown) and human (red) cells in a xenograft model
Lamin B1 (LMNB1) is an intermediate filament-type protein of the nuclear lamina and is ubiquitously expressed throughout development. It plays important roles in many cellular processes like the distribution of heterochromatin and the regulation of gene expression and splicing. The maintenance of LMNB1 protein levels is required for DNA replication and repair and thus mutations in B-type lamins are usually lethal.
Duplication of the LMNB1 gene causes adult-onset autosomal-dominant leukodystrophy (ADLD), a rare neurological disorder in which overexpression of LMNB1 causes progressive central nervous system demyelination. Improper Lamin B1 expression is often present in tumor cells and decreased levels are observed for example in colon cancer, breast cancer and B-cell malignancies. Lamin B1 loss is also a senescence-associated biomarker and distinguishes senescent from proliferating cells in pre-neoplastic lesions or marks senescent cells in various age-related pathologies.