|Cat. No. HS-413 017||200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 gallery
IHC-P/FFPE: 1 : 200 gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 50 to 68 from human CD3e (UniProt Id: P07766)|
Epitop: AA 50 to 68 from human CD3e (UniProt Id: P07766)
Reacts with: human (P07766).
No signal: mouse (P22646).
Other species not tested yet.
|Specificity||Specific for CD3e|
Immunohistochemical staining of T-cells in human tonsil (left) but not in mouse spleen (right) using a human-specific anti-CD3e antibody
Cluster of differentiation 3 (CD3) is a defining feature of cells belonging to the T cell lineage. It is composed of the four subunits CD3 gamma, CD3 delta, CD3 epsilon (CD3e) and CD3 zeta, that form a multimeric protein complex. This complex associates with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 molecules contain immunoreceptor tyrosine-based activation motifs (ITAMs) that serve as the nucleating point for the intracellular signal transduction machinery upon TCR engagement. TCR/CD3 signaling is central to the initiation of antigen-specific T cell responses to pathogens and vaccines, as well as transplanted tissues, tumors, and autoantigens. CD3 is initially expressed in the cytoplasm of pro-thymocytes. During T cell maturation the expression of CD3 migrates to the cell-membrane. The specific appearance at all stages of T cell development make CD3 a useful immunohistochemical marker for T cells in tissue sections. In the clinical setting, CD3 is a relevant marker for the classification of malignant lymphomas and leukemias as the antigen remains present in almost all T-cell lymphomas and leukemias. It can also be used to detect T cells in celiac disease, lymphocytic and collagenous colitis.