|Cat. No. HS-413 108||100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
|Applications||IHC-P/FFPE: 1 : 100 (see remarks) gallery|
|Subtype||IgG1 (κ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 44 to 64 from mouse CD3e (UniProt Id: P22646)|
Epitop: AA 44 to 64 from mouse CD3e (UniProt Id: P22646)
Reacts with: mouse (P22646).
No signal: human (P07766).
Other species not tested yet.
|Specificity||Specific for mouse CD3e without cross-reactivity to human and rat CD3e|
This antibody is a chimeric antibody based on the monoclonal rat antibody clone 19G5B7. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
CD3e positive cells in FFPE mouse liver section
Cluster of differentiation 3 (CD3) is a defining feature of cells belonging to the T cell lineage. It is composed of the four subunits CD3 gamma, CD3 delta, CD3 epsilon (CD3e) and CD3 zeta, that form a multimeric protein complex. This complex associates with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 molecules contain immunoreceptor tyrosine-based activation motifs (ITAMs) that serve as the nucleating point for the intracellular signal transduction machinery upon TCR engagement. TCR/CD3 signaling is central to the initiation of antigen-specific T cell responses to pathogens and vaccines, as well as transplanted tissues, tumors, and autoantigens. CD3 is initially expressed in the cytoplasm of pro-thymocytes. During T cell maturation the expression of CD3 migrates to the cell-membrane. The specific appearance at all stages of T cell development make CD3 a useful immunohistochemical marker for T cells in tissue sections. In the clinical setting, CD3 is a relevant marker for the classification of malignant lymphomas and leukemias as the antigen remains present in almost all T-cell lymphomas and leukemias. It can also be used to detect T cells in celiac disease, lymphocytic and collagenous colitis.