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CD 3e - HS-413 117

Subunit of the T-cell co-receptor
Monoclonal rat purified IgG
Cat. No.: HS-413 117
Amount: 200 µl
Price: 415.00 €
Cat. No. HS-413 117 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C until use.
Applications IHC-P/FFPE: yes gallery  
Clone 19G5B7
Subtype IgG2b (κ light chain)
Immunogen Synthetic peptide corresponding to AA 44 to 64 from mouse CD3e (UniProt Id: P22646)
Epitop Epitop: AA 44 to 64 from mouse CD3e (UniProt Id: P22646)
Reactivity Reacts with: mouse (P22646).
No signal: human (P07766).
Other species not tested yet.
Specificity Specific for mouse CD3e without cross-reactivity to human and rat CD3e.
Data sheet hs-413_117.pdf
Cat. No.: HS-413 117
Quantity: 200 µl
Price: 415.00 €

Double-staining of CD3e positive T-cells and CD19 positive B-cells in FFPE mouse spleen section

Background

Cluster of differentiation 3 (CD3) is a defining feature of cells belonging to the T cell lineage. It is composed of the four subunits CD3 gamma, CD3 delta, CD3 epsilon (CD3e) and CD3 zeta, that form a multimeric protein complex. This complex associates with the T cell receptor (TCR) and serves as a T cell co-receptor. The CD3 molecules contain immunoreceptor tyrosine-based activation motifs (ITAMs) that serve as the nucleating point for the intracellular signal transduction machinery upon TCR engagement. TCR/CD3 signaling is central to the initiation of antigen-specific T cell responses to pathogens and vaccines, as well as transplanted tissues, tumors, and autoantigens. CD3 is initially expressed in the cytoplasm of pro-thymocytes. During T cell maturation the expression of CD3 migrates to the cell-membrane. The specific appearance at all stages of T cell development make CD3 a useful immunohistochemical marker for T cells in tissue sections. In the clinical setting, CD3 is a relevant marker for the classification of malignant lymphomas and leukemias as the antigen remains present in almost all T-cell lymphomas and leukemias. It can also be used to detect T cells in celiac disease, lymphocytic and collagenous colitis.

Protocols