CD45 antibody mouse specific - HS-427 008

CD45 is a large abundant leukocyte-specific cell surface glycoprotein
Rabbit monoclonal recombinant IgG
Cat. No.: HS-427 008
Amount: 100 µl
Price: $415.00
Cat. No. HS-427 008 100 µl purified recombinant IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
IHC-Fr: 1 : 500 MeOH fixation is recommended. (see remarks) gallery  
Clone Rb97A2
Subtype IgG1 (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the carboxy terminus of mouse PTPRC (UniProt Id: P06800-1)
Reactivity Reacts with: mouse (P06800-1).
No signal: human, rat.
Other species not tested yet.
Specificity The antibody recognizes all CD45 isoforms.
Remarks

This antibody is a chimeric antibody based on the monoclonal rat antibody clone GHH45/97A2. The constant regions of the heavy and light chains have been replaced by rabbit specific sequences. Therefore, the antibody can be used with standard anti-rabbit secondary reagents. The antibody has been expressed in mammalian cells.
IHC-Fr: MeOH fixation is recommended.

Data sheet hs-427_008.pdf
Cat. No.: HS-427 008
Amount: 100 µl
Price: $415.00

Detection of CD45+ (red) and F4/80+ (silver-grey) cells in mouse liver

Background

CD45, also designated as Leukocyte Common Antigen (LCA) or Protein tyrosine phosphatase receptor type C (PTPRC), is a type I transmembrane protein expressed on all nucleated cells of the hematopoietic system, except mature erythrocytes and platelets (1). Several isoforms of CD45 have been identified which are generated by differential splicing of exons 4, 5, and 6, thereby generating the CD45RA, RB and RC isoforms, respectively (2). Expression of the isoforms differs according to cell type and functional status. The CD45RABC (B220) long isoform is almost exclusively expressed on B cells. (3). Naïve human T cells express the high molecular weight isoform CD45RA. Activation of T cells and differentiation to memory T cells is accompanied by exon exclusion for production of the short isoform CD45RO (3). CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. Thus, CD45-deficient humans and mice develop a severe-combined immunodeficiency (SCID) phenotype (4). In macrophages, CD45 plays a central role in their adhesion, morphology and motility (5).

Protocols for HS-427 008