|Cat. No. HS-442 017||
100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunocytochemistry (ICC) on 4% PFA fixed cells. Immunoreactivity is usually revealed by fluorescence. Some antibodies require special fixation methods. For details, please refer to the “Remarks” section.', $event)" style="cursor: help;">ICC: 1 : 500 gallery
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 1000 gallery
|Subtype||IgG2b (λ light chain)|
|Immunogen||Synthetic peptide corresponding to AA 275 to 297 from mouse Chil3 (UniProt Id: O35744)|
Reacts with: mouse (O35744).
No signal: human, rat.
Other species not tested yet.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) may improve signal intensity in immunohistochemical stainings.
Chil3 / YM1 positive alveolar macrophages in the mouse lung
Chitinase-3-like protein 3, also referred to as Chil 3, Ym1 and ECF-L, is a secreted protein that is primarily produced by macrophages during inflammation. It is only expressed in rodents, but expression profile and domain organization suggest a functional overlap with human AMCase and SI-CLP. Chil 3 has chemotactic activity for eosinophils, T-cells and polymorphonuclear leukocytes, and plays a role in the inflammatory response elicited by parasitic infections, lung disorders and allergy.
This lectin binds chitin, heparin and GlcN oligomers. Although Chil 3 shares homology with chitinase family proteins, it has no enzymatic activity.