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Chil3 / YM1 antibody - HS-442 017

Chil3/YM1 is a marker protein for murine macrophages
Rat monoclonal purified IgG
Cat. No.: HS-442 017
Amount: 100 µg
Price: $415.00
Cat. No. HS-442 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Applications WB: 1 : 100 up to 1 : 250 gallery  
ICC: 1 : 500 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P/FFPE: 1 : 1000 gallery  
Clone 189C10E2
Subtype IgG2b (λ light chain)
Immunogen Synthetic peptide corresponding to AA 275 to 297 from mouse Chil3 (UniProt Id: O35744)
Epitop Epitop: AA 275 to 297 from mouse Chil3 (UniProt Id: O35744)
Reactivity Reacts with: mouse (O35744).
No signal: human.
Other species not tested yet.
Specificity Specific for murine Chil3 / YM1; no cross-reactivity to Chil4 (YM2)
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) may improve signal intensity in immunohistochemical stainings.

Data sheet hs-442_017.pdf
Cat. No.: HS-442 017
Quantity: 100 µg
Price: $415.00

Chil3 / YM1 positive alveolar macrophages in the mouse lung

Background

Chitinase-3-like protein 3, also referred to as  Chil 3, Ym1 and ECF-L, is a secreted protein that is primarily produced by macrophages during inflammation. It is only expressed in rodents, but expression profile and domain organization suggest a functional overlap with human AMCase and SI-CLP. Chil 3 has chemotactic activity for eosinophils, T-cells and polymorphonuclear leukocytes, and plays a role in the inflammatory response elicited by parasitic infections, lung disorders and allergy.

This lectin binds chitin, heparin and GlcN oligomers. Although Chil 3 shares homology with chitinase family proteins, it has no enzymatic activity.