|Cat. No. HS-452 011||200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
WB: 1 : 1000 gallery
IHC: 1 : 1000 gallery
IHC-P/FFPE: 1 : 1000 gallery
ELISA: yes (see remarks) gallery
|Subtype||IgG2b (κ light chain)|
|Immunogen||Recombinant protein corresponding to AA 1 to 419 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)|
Epitop: AA 16 to 25 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
|Specificity||Specific for nucleocapsid from SARS-CoV2|
ELISA: This antibody is suitable as capture antibody for sandwich-ELISA with cat. no. 452 111BT as detector antibody.
Detection of SARS-CoV-2 nucleocapsid protein in brains of SARS-CoV-2 infected and non-infected K18-hACE2 transgenic mice.
The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other Coronavirus infections.