| Cat. No. HS-452 011BT |
100 µg purified IgG, lyophilized, labeled with
Biotin.
Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use. Antibodies should be stored at +4°C when still lyophilized. Do not freeze! |
| Applications | |
| Label | biotin |
| Clone | 4A8 |
| Subtype | IgG2b (κ light chain) |
| Specificity | Specific for nucleocapsid from SARS-CoV2 |
| Remarks |
ELISA: For a higher sensitivity in sandwich ELISA we recommend the use of cat. no. HS-452 011 as capture antibody and HS-cat. no. 452 111BT as detector antibody |
| Data sheet | hs-452_011bt.pdf |
Detection of SARS-CoV-2 nucleocapsid protein in the brain of a SARS-CoV-2 infected K18-hACE2 transgenic mouse.
The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other coronavirus infections.