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Nucleocapsid CoV-1/2 antibody - HS-452 111

CoV2 protein N packages the viral genome RNA
Mouse monoclonal purified IgG
Cat. No.: HS-452 111
Amount: 200 µl
Price: $415.00
Cat. No. HS-452 111 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
ICC: yes gallery  
IHC: 1 : 500 up to 1 : 1000 gallery  
IHC-P: 1 : 1000 gallery  
ELISA: yes
Clone 53E2
Subtype IgG2a (κ light chain)
Immunogen Recombinant protein corresponding to AA 1 to 419 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
Epitop Epitop: AA 401 to 410 from SARS-Cov-2 Nucleocapsid protein (UniProt Id: P0DTC9)
Specificity Specific for nucleocapsid from SARS-CoV1 and SARS-CoV2
Data sheet hs-452_111.pdf

References for Nucleocapsid CoV-1/2 - HS-452 111

Cardiac SARS-CoV-2 infection is associated with pro-inflammatory transcriptomic alterations within the heart.
Bräuninger H, Stoffers B, Fitzek ADE, Meißner K, Aleshcheva G, Schweizer M, Weimann J, Rotter B, Warnke S, Edler C, Braun F, et al.
Cardiovascular research (2022) 1182: 542-555. HS-452 111 IHC-P; tested species: human
Assessing and improving the validity of COVID-19 autopsy studies - A multicentre approach to establish essential standards for immunohistochemical and ultrastructural analyses.
Krasemann S, Dittmayer C, von Stillfried S, Meinhardt J, Heinrich F, Hartmann K, Pfefferle S, Thies E, von Manitius R, Aschman TAD, Radke J, et al.
EBioMedicine (2022) 83: 104193. HS-452 111 IHC-P; tested species: human
Cat. No.: HS-452 111
Amount: 200 µl
Price: $415.00

Detection of SARS-CoV-2 nucleocapsid protein in brains of SARS-CoV-2  infected and non-infected K18-hACE2 transgenic mice.

Cardiac SARS-CoV-2 infection is associated with pro-inflammatory transcriptomic alterations within the heart.
Bräuninger H, Stoffers B, Fitzek ADE, Meißner K, Aleshcheva G, Schweizer M, Weimann J, Rotter B, Warnke S, Edler C, Braun F, et al.
Cardiovascular research (2022) 1182: 542-555. HS-452 111 IHC-P; tested species: human
Assessing and improving the validity of COVID-19 autopsy studies - A multicentre approach to establish essential standards for immunohistochemical and ultrastructural analyses.
Krasemann S, Dittmayer C, von Stillfried S, Meinhardt J, Heinrich F, Hartmann K, Pfefferle S, Thies E, von Manitius R, Aschman TAD, Radke J, et al.
EBioMedicine (2022) 83: 104193. HS-452 111 IHC-P; tested species: human
Background

The severe acute respiratory syndrome coronavirus type 2 (SARS-CoV2) is an enveloped positive-sense single stranded RNA virus that has been identified in the beginning of 2020 (1). It infects human host cells by docking via its spike protein (S) to the ACE2 surface receptor (2) and can cause mild to very severe and even deadly Covid-19 courses (3).
The very abundant Nucleocapsid or N-protein packages the viral RNA (4) and shares only little homology to other abundant members of the coronavirus family like NL63, 229E, HKU1 or OC43. This characteristic makes it a suitable target to discriminate between Covid-19 and other Coronavirus infections.

Protocols for HS-452 111