|Cat. No. HS-455 003||
200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 250 gallery
|Immunogen||Synthetic peptide corresponding to AA 870 to 888 from mouse CD163 (UniProt Id: Q2VLH6)|
Reacts with: mouse (Q2VLH6).
No signal: human.
Other species not tested yet.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.
Indirect immunostaining of mouse spleen section with rabbit anti-CD163 (red) and Guinea pig anti-IBA1 (green).
CD163 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily expressed on human and murine macrophages. CD163 is also expressed in human monocytes, but not in circulating mouse monocytes (1). CD163 expression is significantly induced by anti-inflammatory stimuli like glucocorticoids and dexamethasone. Inflammatory stimuli like interferon γ and LPS suppress CD163 expression in macrophages (2). In mice, CD163 is mainly expressed by tissue-resident macrophages including Kupffer cells of the liver, red pulp macrophages in the spleen, perivascular macrophages of the CNS (3), and bone-marrow resident macrophages (4), but not by classical bone-marrow derived macrophages (4). In malignant tumors in mice and humans, macrophage CD163-mediated induction of IL-6 promotes tumor development and progression (4). The pool of tumor-associated macrophages (TAMs) is composed of both newly recruited monocyte derived macrophages and resident macrophages (5). CD163-positive tissue-resident macrophages have been shown to play a specific role in the malignant spread of disseminated tumor cells and the development of invasive disease in a mouse model of metastatic ovarian cancer (1). CD163 is considered useful to distinguish CD163-positive resident macrophages from CD163-negative bone-marrow derived macrophages in mice (4).