|Cat. No. HS-455 003||200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.|
IHC: 1 : 125 (see remarks) gallery
IHC-P/FFPE: 1 : 250 gallery
|Immunogen||Synthetic peptide corresponding to AA 870 to 888 from mouse CD163 (UniProt Id: Q2VLH6)|
Reacts with: mouse (Q2VLH6).
No signal: human.
Other species not tested yet.
|Specificity||Specific for CD163|
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.
Indirect immunostaining of mouse spleen section with rabbit anti-CD163 (red) and Guinea pig anti-IBA1 (green).
CD163 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily which is expressed on human and murine macrophages. CD163 is also expressed in human monocytes, but not in circulating mouse monocytes (1). CD163 expression is significantly induced by anti-inflammatory stimuli like glucocorticoids and dexamethasome (2,3). Inflammatory stimuli like interferon γ and LPS suppress CD163 expression in macrophages (2, 3). In mice, CD163 is mainly expressed by tissue-resident macrophages (4) including Kupffer cells of the liver, red pulp macrophages of the spleen, perivascular macrophages of the CNS (5) and bone-marrow resident macrophages (6), but not by classical bone-marrow derived macrophages (6,7). In murine and human malignant tumors, macrophage CD163-mediated induction of IL-6 promotes tumor development and progression (7). The pool of tumor-associated macrophages (TAMs) is composed of both newly recruited monocyte derived macrophages and resident macrophages (8). CD163 positive tissue-resident macrophages have been shown to play a specific role in the malignant progression of disseminated tumor cells and the development of invasive disease in a mouse model of metastatic ovarian cancer (1). CD163 is considered useful to distinguish CD163-positive resident macrophages from CD163-negative bone-marrow derived macrophages in mice (7).