CD163 antibody mouse specific - HS-455 003

The scavenger receptor CD163 is a marker of cells from the monocyte / macrophage lineage
Rabbit polyclonal purified antibody
Cat. No.: HS-455 003
Amount: 200 µl
Price: $370.00
Cat. No. HS-455 003 200 µl specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
IHC: 1 : 125 (see remarks) gallery  
IHC-P: 1 : 250 gallery  
Immunogen Synthetic peptide corresponding to AA 870 to 888 from mouse CD163 (UniProt Id: Q2VLH6)
Reactivity Reacts with: mouse (Q2VLH6).
No signal: human.
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet hs-455_003.pdf
Cat. No.: HS-455 003
Amount: 200 µl
Price: $370.00

Indirect immunostaining of mouse spleen section with rabbit anti-CD163 (red) and Guinea pig anti-IBA1 (green).

Background

CD163 is a member of the group B scavenger receptor cysteine-rich (SRCR) superfamily expressed on human and murine macrophages. CD163 is also expressed in human monocytes, but not in circulating mouse monocytes (1). CD163 expression is significantly induced by anti-inflammatory stimuli like glucocorticoids and dexamethasone. Inflammatory stimuli like interferon γ and LPS suppress CD163 expression in macrophages (2). In mice, CD163 is mainly expressed by tissue-resident macrophages including Kupffer cells of the liver, red pulp macrophages in the spleen, perivascular macrophages of the CNS (3), and bone-marrow resident macrophages (4), but not by classical bone-marrow derived macrophages (4). In malignant tumors in mice and humans, macrophage CD163-mediated induction of IL-6 promotes tumor development and progression (4). The pool of tumor-associated macrophages (TAMs) is composed of both newly recruited monocyte derived macrophages and resident macrophages (5). CD163-positive tissue-resident macrophages have been shown to play a specific role in the malignant spread of disseminated tumor cells and the development of invasive disease in a mouse model of metastatic ovarian cancer (1). CD163 is considered useful to distinguish CD163-positive resident macrophages from CD163-negative bone-marrow derived macrophages in mice (4).

Protocols for HS-455 003