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CD68 antibody - HS-460 017

CD68 is a marker for macrophages and activated microglia
Monoclonal rat purified IgG
Cat. No.: HS-460 017
Amount: 200 µl
Price: $415.00
Cat. No. HS-460 017 200 µl purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 200 µl H2O. Then aliquot and store at -20°C to -80°C until use.
Applications ICCgallery  
IHC-P/FFPE: yes gallery  
Clone 186F9B4
Subtype IgG2b (κ light chain)
Epitop Epitop: AA 345 to 354 from human CD68 (UniProt Id: P34810)
Reactivity Reacts with: human (P34810).
No signal: mouse (P31996).
Other species not tested yet.
Specificity Specific for human CD68
Data sheet hs-460_017.pdf
Cat. No.: HS-460 017
Quantity: 200 µl
Price: $415.00

Indirect immunostaining of human tonsil section with rat anti-CD68 (red) and rabbit anti-IBA1 (green).

Background

CD68, also called Lamp-4 in humans or macrosialin in mice, is a highly glycosylated type I transmembrane protein that belongs to the lysosome associated membrane protein (LAMP) family (1). CD68 expression is increased in cells associated with elevated phagocytic and degradative activity. High CD68 expression is detected in cells of the mononuclear phagocyte lineage including macrophages, osteoclasts, and myeloid dendritic cells (2). CD68 is a marker of activated microglia and only expressed at low levels in resting microglia (3). Staining for CD68 is predominantly intracellular, only 10 -15% of it is found on the cell surface. In oncology research, CD68 is the major biomarker for quantification of tumor-associated macrophages (TAMs) (4). High infiltration of CD68+ macrophages is an independent prognostic factor for overall survival in several tumor entities (5).

Protocols
IHC-P