CD86 antibody mouse specific - HS-466 003

CD86 is a costimulatory molecule expressed by antigen-presenting cells
Rabbit polyclonal purified antibody
Cat. No.: HS-466 003
Amount: 50 µg
Price: $370.00
Cat. No. HS-466 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 250 gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 400 gallery  
Immunogen Synthetic peptide corresponding to AA 291 to 309 from mouse Cd86 (UniProt Id: P42082)
Reactivity Reacts with: mouse (P42082).
No signal: human (P42081), rat.
Other species not tested yet.

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining of PFA fixed tissues. Methanol fixation is required for immunohistochemical staining of fresh frozen tissues.

Data sheet hs-466_003.pdf
Cat. No.: HS-466 003
Amount: 50 µg
Price: $370.00

CD86 positive cells in a Toxoplasmose gondii infected mouse liver section


CD86 (Cluster of Differentiation 86, also known as B7.2) belongs to the B7 family of immune-regulatory cell-surface protein ligands (1). CD86 and the genetically closely linked CD80 protein (also known as B7.1) are expressed by antigen presenting cells and provide costimulatory signals necessary for T cell activation and tolerance via interaction with CD28 and cytotoxic T-lymphocyte antigen 4 (CTLA-4) expressed on T-cells. However, CD80 and CD86 have non-equivalent roles in immune modulation: CD86 is the dominant ligand for proliferation and survival of regulatory T cells (Tregs) (2) and shows in comparison with CD80 very high efficiency at increasing T cell killing capacity (3). CD86 is expressed only at low levels on resting B cells, dendritic cells and macrophages; activation results in enhanced CD86 expression (Collins et al., 2005). In the CNS, CD86 upregulation is a marker of activated pro-inflammatory M1 microglia (4). In oncology research, CD86 is a biomarker to phenotypically differentiate classically activated M1 macrophages from alternatively activated M2 macrophages in the tumor microenvironment (5).

Protocols for HS-466 003