E-cadherin antibody mouse specific - HS-467 003

E-cadherin is a cell adhesion protein
Rabbit polyclonal purified antibody
Cat. No.: HS-467 003
Amount: 50 µg
Price: $370.00
Cat. No. HS-467 003 50 µg specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 gallery  
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 100 up to 1 : 250 gallery  
Reactivity Reacts with: mouse (P09803), rat.
No signal: human.
Other species not tested yet.
Remarks

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.

Data sheet hs-467_003.pdf
Cat. No.: HS-467 003
Amount: 50 µg
Price: $370.00

Indirect immunostaining of human and mouse duodenum sections using mouse-E-cadherin specific antibody.

Background

Epithelial cadherin (E-cadherin) also known as Cadherin-1, CAM 120/80 or uvomorulin belongs together with neuronal (N) cadherin to the type I classical cadherins, transmembrane proteins that function in calcium-dependent cell-cell adhesion (1). In normal tissues, E-cadherin is expressed by most epithelial cells. A distinct distribution of E-cadherin expression is found in the kidney, where only distal tubuli show E-cadherin expression and in the placenta, where only the cytotrophoblastic layer stains positive for E-cadherin (2). In the human normal adult nervous system E-cadherin expression is limited to the arachnoid membrane, whereas in mice E-cadherin is also expressed in neural stem cells, where E-cadherin regulates self-renewal (3). E-cadherin is a potent tumor suppressor and the so-called “cadherin switch” - downregulation of E-cadherin while N-cadherin is upregulated - is often found in malignant epithelial cancers. This Epithelial-to-Mesenchymal Transition (EMT) has been shown to be crucial in tumorigenesis where the EMT program enhances metastasis, chemoresistance and tumor stemness (4). However, also E-cadherin upregulation in malignancies derived from E-cadherin negative normal tissues tend to be linked to unfavorable tumor phenotype and disease outcome (2). In syngeneic mouse tumors E-cadherin expression is typically lower than in human tumors suggesting that syngeneic mouse models have a more mesenchymal-like tumor cellular phenotype (5).

Protocols for HS-467 003