|Cat. No. HS-470 004||
100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Immunohistochemistry (IHC) on 4% PFA perfusion fixed tissue with 24h PFA post fixation. Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate. Some antibodies require special fixation methods or antigen retrieval steps. For details, please refer to the ”Remarks” section.', $event)" style="cursor: help;">IHC: 1 : 500 (see remarks) gallery
Immunohistochemistry (IHC-P) of formalin fixed, paraffin embedded (FFPE) tissue (some antibodies require special antigen retrieval steps, please refer to the ”Remarks” section). Immunoreactivity is usually revealed by fluorescence or a chromogenic substrate.', $event)" style="cursor: help;">IHC-P: 1 : 500 up to 1 : 750 gallery
|Immunogen||Synthetic peptide corresponding to AA 108 to 128 from mouse Vcam1 (UniProt Id: Q3UPN1)|
Reacts with: mouse (Q3UPN1), rat (P29534).
No signal: human (P19320).
Other species not tested yet.
IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical stainings.
Immunohistochemical analysis of paraffin-embedded mouse spleen using VCAM-1 (mouse and rat specific)
Vascular cell adhesion molecule 1 (Vcam1 or CD106) is an endothelial adhesion molecule of the Ig gene superfamily, that regulates leukocyte migration from the blood into tissues (1). Expression of Vcam1 is induced on endothelial cells during inflammatory diseases in response to a number of inflammatory stimuli, including TNFα, lipopolysaccharide or high levels of reactive oxygen species (2). Vcam1 expression in hematopoietic cells is restricted to cells confined to tissues e.g., red pulp macrophages and follicular dendritic cells in the spleen, whereas circulating cells are virtually devoid of Vcam1 (3). Vcam1 has been found to be implicated in various immunological disorders, including rheumatoid arthritis, transplant rejection and cancer and is therefore a potential therapeutic target (4).