VCAM1 antibody mouse specific - HS-470 004

VCAM1 is expressed by cytokine activated endothelium
Guinea pig polyclonal antiserum
Cat. No.: HS-470 004
Amount: 100 µl
Price: $350.00
Cat. No. HS-470 004 100 µl antiserum, lyophilized. For reconstitution add 100 µl H2O, then aliquot and store at -20°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
WB: 1 : 1000 (AP-staining) gallery  
IP: not tested yet
ICC: not tested yet
IHC: 1 : 500 (see remarks) gallery  
IHC-P: 1 : 500 up to 1 : 750 gallery  
Immunogen Synthetic peptide corresponding to AA 108 to 128 from mouse Vcam1 (UniProt Id: Q3UPN1)
Reactivity Reacts with: mouse (Q3UPN1), rat (P29534).
No signal: human (P19320).
Other species not tested yet.

IHC: Heat-mediated antigen retrieval (in citrate buffer pH 6) is required for immunohistochemical staining.

Data sheet hs-470_004.pdf
Cat. No.: HS-470 004
Amount: 100 µl
Price: $350.00

Immunohistochemical analysis of paraffin-embedded mouse spleen using VCAM-1 (mouse and rat specific)


Vascular cell adhesion molecule 1 (Vcam1 or CD106) is an endothelial adhesion molecule of the Ig gene superfamily, that regulates leukocyte migration from the blood into tissues (1). Expression of Vcam1 is induced on endothelial cells during inflammatory diseases in response to a number of inflammatory stimuli, including TNFα, lipopolysaccharide or high levels of reactive oxygen species (2). Vcam1 expression in hematopoietic cells is restricted to cells confined to tissues e.g., red pulp macrophages and follicular dendritic cells in the spleen, whereas circulating cells are virtually devoid of Vcam1 (3). Vcam1 has been found to be implicated in various immunological disorders, including rheumatoid arthritis, transplant rejection and cancer and is therefore a potential therapeutic target (4).

Protocols for HS-470 004