Galectin-3 antibody mouse specific - HS-477 017 K.O.

Galectin-3 is a β-galactoside-binding lectin involved in host defense
Rat monoclonal purified IgG
Cat. No.: HS-477 017
Amount: 100 µg
Price: $415.00
Cat. No. HS-477 017 100 µg purified IgG, lyophilized. Albumin and azide were added for stabilization. For reconstitution add 100 µl H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C to -80°C until use.
Antibodies should be stored at +4°C when still lyophilized. Do not freeze!
Applications
 
WB: 1 : 1000 (AP-staining) gallery  
IHC: 1 : 500 gallery  
IHC-P: 1 : 1000 gallery  
IHC-Fr: 1 : 500 (see remarks) gallery  
Clone SY-17F7
Subtype IgG2a (κ light chain)
Immunogen Synthetic peptide corresponding to residues near the amino terminus of mouse Galectin-3 (UniProt Id: P16110)
Reactivity Reacts with: mouse (P16110).
No signal: human, rat.
Other species not tested yet.
Specificity K.O. validated
Remarks

IHC-Fr: PFA fixation is recommended.

Data sheet hs-477_017.pdf
Cat. No.: HS-477 017
Amount: 100 µg
Price: $415.00

Galectin-3 is expressed in red pulp macrophages in the spleen

Background

Galectin-3 (Gal-3, Mac-2) is a β-galactosidase binding protein that is predominantly located in the cytoplasm and shuttles into the nucleus. In addition, it is secreted to the cell surface and into biological fluids. Depending on its localization, it exerts pleiotropic biological functions like cell growth, cell differentiation, inflammation, fibrogenesis, migration, apoptosis and host defense (1). It is also involved in cancer pathogenesis, proliferation and spreading of metastasis (2). Galectin-3 is highly expressed in the covering epithelia of the digestive tract and the urinary system (3) and plays important roles in the organization of renal and intestinal cells. Furthermore, Galectin-3 has been identified in a variety of other cell types including fibroblasts, keratinocytes, granulocytes, dendritic cells and macrophages (2). Galectin-3 is expressed by certain tissue-resident macrophages such as Kupffer cells in the liver, red pulp macrophages in the spleen and alveolar macrophages in the lung (3). In the brain, Galectin-3 is abundant in activated microglia but not expressed in resting cells. Following brain injury, Galectin-3 triggers anti-inflammatory properties of microglia (4). In Alzheimer’s disease, Gal-3 expression is strictly associated with microglial cell activation around Aβ; plaques. In stroke Gal-3 was found to be expressed primarily in proinflammatory microglial cells (5).